ArticleThe EMBO journal2007
The restriction fold turns to the dark side: a bacterial homing endonuclease with a PD-(D/E)-XK motif.
Article in The EMBO journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- Filament assembly induced by the recognition of target DNA activates the prokaryotic Argonaute SPARDA system.Nature communications · 2026Article
- Activation of the SPARDA defense system by filament assembly using a beta-relay signaling mechanism widespread in prokaryotic Argonautes.Cell research · 2025Article
- Target DNA-induced filament formation and nuclease activation of SPARDA complex.Cell research · 2025Article
- Biosafety considerations triggered by genome-editing technologies.Biosafety and health · 2025Review
- The nuclease-associated short prokaryotic Argonaute system nonspecifically degrades DNA upon activation by target recognition.Nucleic acids research · 2024Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.NAR genomics and bioinformatics · 2023Article
- Nuclease genes occupy boundaries of genetic exchange between bacteriophages.bioRxiv : the preprint server for biology · 2023Article
- Post-genomic era in agriculture and veterinary science: successful and proposed application of genetic targeting technologies.Frontiers in veterinary science · 2023Review
- Genome-Wide Identification and Transcriptional Analysis of ArabidopsisInternational journal of molecular sciences · 2021Article
- F-CphI represents a new homing endonuclease family using the Endo VII catalytic motif.Mobile DNA · 2018Article
- Rpn (YhgA-Like) Proteins of Escherichia coli K-12 and Their Contribution to RecA-Independent Horizontal Transfer.Journal of bacteriology · 2017Article
- Genomics Approaches For Improving Salinity Stress Tolerance in Crop Plants.Current genomics · 2016Article
- Single-Cell (Meta-)Genomics of a Dimorphic Candidatus Thiomargarita nelsonii Reveals Genomic Plasticity.Frontiers in microbiology · 2016Article
- Structural characterization of MepB from Staphylococcus aureus reveals homology to endonucleases.Protein science : a publication of the Protein Society · 2014Article
- Article
- Homing endonucleases: from genetic anomalies to programmable genomic clippers.Methods in molecular biology (Clifton, N.J.) · 2014Review
- Sequence, structure and functional diversity of PD-(D/E)XK phosphodiesterase superfamily.Nucleic acids research · 2012Article
- Structural, functional and evolutionary relationships between homing endonucleases and proteins from their host organisms.Nucleic acids research · 2012Review
- Polymorphic toxin systems: Comprehensive characterization of trafficking modes, processing, mechanisms of action, immunity and ecology using comparative genomics.Biology direct · 2012Article
- Gene flow and biological conflict systems in the origin and evolution of eukaryotes.Frontiers in cellular and infection microbiology · 2012Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The homing endonuclease I-Ssp6803I causes the insertion of a group I intron into a bacterial tRNA gene-the only example of an invasive mobile intron within a bacterial genome. Using a computational fold prediction, mutagenic screen and crystal structure determination, we demonstrate that this protein is a tetrameric PD-(D/E)-XK endonuclease - a fold normally used to protect a bacterial genome from invading DNA through the action of restriction endonucleases. I-Ssp6803I uses its tetrameric assembly to promote recognition of a single long target site, whereas restriction endonuclease tetramers facilitate cooperative binding and cleavage of two short sites. The limited use of the PD-(D/E)-XK nucleases by mobile introns stands in contrast to their frequent use of LAGLIDADG and HNH endonucleases - which in turn, are rarely incorporated into restriction/modification systems.
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