ArticleGene therapy2007
Sensitive and precise regulation of haemoglobin after gene transfer of erythropoietin to muscle tissue using electroporation.
Article in Gene therapy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Exosomes as an Emerging Plasmid Delivery Vehicle for Gene Therapy.Pharmaceutics · 2023Review
- Optimization of Mouse Growth Hormone Plasmid DNA Electrotransfer into Tibialis Cranialis Muscle of "Little" Mice.Molecules (Basel, Switzerland) · 2020Article
- Induction of a local muscular dystrophy using electroporation in vivo: an easy tool for screening therapeutics.Scientific reports · 2020Article
- A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle.International journal of molecular sciences · 2019Review
- Optimizing hyaluronidase dose and plasmid DNA delivery greatly improves gene electrotransfer efficiency in rat skeletal muscle.Biochemistry and biophysics reports · 2015Article
- Article
- Electroporation-mediated gene delivery.Advances in genetics · 2015Article
- What you always needed to know about electroporation based DNA vaccines.Human vaccines & immunotherapeutics · 2012Review
- A combination of intradermal jet-injection and electroporation overcomes in vivo dose restriction of DNA vaccines.Genetic vaccines and therapy · 2012Article
- Human erythropoietin gene delivery using an arginine-grafted bioreducible polymer system.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Comparison of plasmid vaccine immunization schedules using intradermal in vivo electroporation.Clinical and vaccine immunology : CVI · 2011Article
- Exercise-induced liver chemokine CXCL-1 expression is linked to muscle-derived interleukin-6 expression.The Journal of physiology · 2011Article
- Direct and long-term detection of gene doping in conventional blood samples.Gene therapy · 2011Article
- Minimal doses of a sequence-optimized transgene mediate high-level and long-term EPO expression in vivo: challenging CpG-free gene design.Gene therapy · 2011Article
- Novel serum biomarkers for erythropoietin use in humans: a proteomic approach.Journal of applied physiology (Bethesda, Md. : 1985) · 2011Article
- Therapeutic levels of erythropoietin (EPO) achieved after gene electrotransfer to skin in mice.Gene therapy · 2010Article
- Duration and level of transgene expression after gene electrotransfer to skin in mice.Gene therapy · 2010Article
- Change in Hemoglobin Levels due to Anesthesia in Mice: An Important Confounder in Studies on Hematopoietic Drugs.Biological procedures online · 2009Article
- Gene electrotransfer: from biophysical mechanisms to in vivo applications : Part 2 - In vivo developments and present clinical applications.Biophysical reviews · 2009Review
- Erythropoietin over-expression protects against diet-induced obesity in mice through increased fat oxidation in muscles.PloS one · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Electroporation-based gene transfer (electro gene transfer (EGT)) is gaining increasing momentum, in particular for muscle tissue, where long-term high-level expression is obtainable. Induction of expression using the Tet-On system was previously established; however, attempts to reach a predefined target dose - a prescription, have not been reported. We set three target haemoglobin levels (10, 12 and 14 mmol/l, base level was 8.2 mmol/l) and aimed at them by transferring the erythropoietin (EPO) gene to mouse tibialis cranialis (TC) muscle, and varying (1) DNA amount, (2) muscle mass transfected and (3) induction with the Tet-On system. Results showed that (a) using GFP, luciferase and EPO low DNA amounts were needed. In fact, 0.5 microg of DNA to one TC muscle led to significant Hgb elevation - this amount extrapolates to 1.4 mg of DNA in humans, (b) three prescribers hit the targets with average Hgb of 10.5, 12.0 and 13.7 mmol/l, (c) different approaches could be used, (d) undershooting could be corrected by retransferring, and (e) overshooting could be alleviated by reducing dose of inducer (doxycycline (dox)). In conclusion, this study shows that using EGT to muscle, a preset level of protein expression can be reached. This is of great interest for future clinical use.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.