ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2007
Effects of detergents on the redistribution of gangliosides and GPI-anchored proteins in brain tissue sections.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?Biomolecules · 2024Review
- Who's in, who's out? Re-evaluation of lipid raft residents.Journal of neurochemistry · 2021Article
- Neuronal Signaling by Thy-1 in Nanodomains With Specific Ganglioside Composition: Shall We Open the Door to a New Complexity?Frontiers in cell and developmental biology · 2019Article
- Folate receptor 1 is necessary for neural plate cell apical constriction duringDevelopment (Cambridge, England) · 2017Article
- Detection of brain-directed autoantibodies in the serum of non-small cell lung cancer patients.PloS one · 2017Article
- Gangliosides of the Vertebrate Nervous System.Journal of molecular biology · 2016Review
- δ-Opioid receptors: Pivotal role in intermittent hypoxia-augmentation of cardiac parasympathetic control and plasticity.Autonomic neuroscience : basic & clinical · 2016Article
- Nfasc155H and MAG are specifically susceptible to detergent extraction in the absence of the myelin sphingolipid sulfatide.Neurochemical research · 2013Article
- Differential distribution of major brain gangliosides in the adult mouse central nervous system.PloS one · 2013Article
- The TvLEGU-1, a legumain-like cysteine proteinase, plays a key role in Trichomonas vaginalis cytoadherence.BioMed research international · 2013Article
- Biosynthesis of the major brain gangliosides GD1a and GT1b.Glycobiology · 2012Article
- Article
- Isolation of rafts from mouse brain tissue by a detergent-free method.Journal of lipid research · 2009Article
- Reverse signaling by glycosylphosphatidylinositol-linked Manduca ephrin requires a SRC family kinase to restrict neuronal migration in vivo.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Membrane-targeted strategies for modulating APP and Abeta-mediated toxicity.Journal of cellular and molecular medicine · 2009Review
- Immunohistochemical markers for quantitative studies of neurons and glia in human neocortex.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2008Article
- Thy-1 is expressed in hepatic myofibroblasts and not oval cells in stem cell-mediated liver regeneration.The American journal of pathology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
Abstract
Gangliosides and glycosylphosphatidylinositol (GPI)-anchored proteins contain lipid tails that tether them to the outer side of the cell membrane. This mode of association with the cell membrane enables them to take part in the organization of lipid rafts, but it also permits gangliosides and GPI-anchored proteins to be actively released from one cell and inserted into the membrane of another cell. Recently, we reported that under conditions of lipid raft isolation, Triton X-100 causes significant redistribution of both gangliosides and GPI-anchored proteins. Aiming to find a less disruptive detergent, we evaluated the effects of CHAPS, Saponin, deoxycholic acid, Trappsol, Tween 20, Triton X-100, Brij 96V, Brij 98, and SDS on brain tissue sections. At room temperature, all detergents (1% concentration) extracted significant amounts of both gangliosides and Thy-1. At 4C, the extraction was weaker, but Triton X-100, CHAPS, and deoxycholic acid caused significant redistribution of GD1a and Thy-1 from gray matter into the white matter. Both redistribution and extraction were significantly augmented when sections were incubated with detergents in the presence of primary antibodies. Of the nine tested detergents, none is the ideal choice. However, Brij 96V appears to be able to sufficiently reveal myelin epitopes while causing the least amount of artifacts. This manuscript contains online supplemental material at http://www.jhc.org. Please visit this article online to view these materials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.