ArticleMolecular biology of the cell2007
Receptor complexes cotransported via polarized endocytic pathways form clusters with distinct organizations.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
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- Quantitative imaging of receptor-ligand engagement in intact live animals.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- TOWARD BAYESIAN INFERENCE OF THE SPATIAL DISTRIBUTION OF PROTEINS FROM THREE-CUBE FÖRSTER RESONANCE ENERGY TRANSFER DATA.The annals of applied statistics · 2017Article
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- Antigen-B Cell Receptor Complexes Associate with Intracellular major histocompatibility complex (MHC) Class II Molecules.The Journal of biological chemistry · 2015Article
- Single-molecule analyses of fully functional fluorescent protein-tagged follitropin receptor reveal homodimerization and specific heterodimerization with lutropin receptor.Biology of reproduction · 2015Article
- Temporal Data Set Reduction Based on D-Optimality for Quantitative FLIM-FRET Imaging.PloS one · 2015Article
- Reduced temporal sampling effect on accuracy of time-domain fluorescence lifetime Förster resonance energy transfer.Journal of biomedical optics · 2014Article
- Automated selection of regions of interest for intensity-based FRET analysis of transferrin endocytic trafficking in normal vs. cancer cells.Methods (San Diego, Calif.) · 2014Article
- IQGAP1 interactome analysis by in vitro reconstitution and live cell 3-color FRET microscopy.Cytoskeleton (Hoboken, N.J.) · 2013Article
- Active wide-field illumination for high-throughput fluorescence lifetime imaging.Optics letters · 2013Article
- Förster resonance energy transfer microscopy and spectroscopy for localizing protein-protein interactions in living cells.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2013Review
- Non-invasive in vivo imaging of near infrared-labeled transferrin in breast cancer cells and tumors using fluorescence lifetime FRET.PloS one · 2013Article
- Three-Color FRET expands the ability to quantify the interactions of several proteins involved in actin filament nucleation.Proceedings of SPIE--the International Society for Optical Engineering · 2012Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Previously, FRET confocal microscopy has shown that polymeric IgA-receptor (pIgA-R) is distributed in a clustered manner in apical endosomes. To test whether different membrane-bound components form clusters during membrane trafficking, live-cell quantitative FRET was used to characterize the organization of pIgA-R and transferrin receptor (TFR) in endocytic membranes of polarized MDCK cells upon internalization of donor- and acceptor-labeled ligands. We show that pIgA-R and TFR complexes form increasingly organized clusters during cotransport from basolateral to perinuclear endosomes. The organization of these receptor clusters in basolateral versus perinuclear/apical endosomes is significantly different; the former showing a mixed random/clustered distribution while the latter highly organized clusters. Our results indicate that although both perinuclear and apical endosomes comprise pIgA-R and TFR clusters, their E% levels are significantly different suggesting that these receptors are packed into clusters in a distinct manner. The quantitative FRET-based assay presented here suggests that different receptor complexes form clusters, with diverse levels of organization, while being cotransported via the polarized endocytic pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.