ArticleJournal of molecular biology2007
Binding of ATP to TK1-like enzymes is associated with a conformational change in the quaternary structure.
Article in Journal of molecular biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 36 citations in OpenAlex.
- Inactive-to-Active Transition of Human Thymidine Kinase 1 Revealed by Molecular Dynamics Simulations.Journal of chemical information and modeling · 2022Article
- Thymidine kinase 1 through the ages: a comprehensive review.Cell & bioscience · 2020Review
- Evaluation of [Molecular imaging and biology · 2017Article
- New Variants of Tomato Thymidine Kinase 1 Selected for Increased Sensitivity of E. coli KY895 towards Azidothymidine.Cancers · 2015Article
- Structural and Kinetic Characterization of Thymidine Kinase from Leishmania major.PLoS neglected tropical diseases · 2015Article
- KAY-2-41, a novel nucleoside analogue inhibitor of orthopoxviruses in vitro and in vivo.Antimicrobial agents and chemotherapy · 2014Article
- FISH comets show that the salvage enzyme TK1 contributes to gene-specific DNA repair.Frontiers in genetics · 2014Article
- N3-substituted thymidine bioconjugates for cancer therapy and imaging.Future medicinal chemistry · 2013Review
- Synthesis of N3-substituted carboranyl thymidine bioconjugates and their evaluation as substrates of recombinant human thymidine kinase 1.European journal of medicinal chemistry · 2013Article
- Quaternary structures of recombinant, cellular, and serum forms of thymidine kinase 1 from dogs and humans.BMC biochemistry · 2012Article
- Synthesis, biological evaluation, and radioiodination of halogenated closo-carboranylthymidine analogues.Inorganic chemistry · 2012Article
- Isolation and characterization of a Mn(II)-oxidizing Bacillus strain from the demosponge Suberites domuncula.Marine drugs · 2010Article
- Structural and kinetic characterization of human deoxycytidine kinase variants able to phosphorylate 5-substituted deoxycytidine and thymidine analogues .Biochemistry · 2010Article
- Engineering Kinases to Phosphorylate Nucleoside Analogs for Antiviral and Cancer Therapy.Chimia · 2009Article
- Computational molecular biology approaches to ligand-target interactions.HFSP journal · 2009Article
- Directed evolution of an orthogonal nucleoside analog kinase via fluorescence-activated cell sorting.Nucleic acids research · 2009Article
- Extending thymidine kinase activity to the catalytic repertoire of human deoxycytidine kinase.Biochemistry · 2009Article
- Quaternary structure change as a mechanism for the regulation of thymidine kinase 1-like enzymes.Structure (London, England : 1993) · 2007Article
- Exploiting temperature-dependent substrate promiscuity for nucleoside analogue activation by thymidine kinase from Thermotoga maritima.Journal of the American Chemical Society · 2007Article
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
Human thymidine kinase 1 (hTK1) and structurally related TKs from other organisms catalyze the initial phosphorylation step in the thymidine salvage pathway. Though ATP is known to be the preferred phosphoryl donor for TK1-like enzymes, its exact binding mode and effect on the oligomeric state has not been analyzed. Here we report the structures of hTK1 and of the Thermotoga maritima thymidine kinase (TmTK) in complex with the bisubstrate inhibitor TP4A. The TmTK-TP4A structure reveals that the adenosine moiety of ATP binds at the subunit interface of the homotetrameric enzyme and that the majority of the ATP-enzyme interactions occur between the phosphate groups and the P-loop. In the hTK1 structure the adenosine group of TP4A exhibited no electron density. This difference between hTK1 and TmTK is rationalized by a difference in the conformation of their quaternary structure. A more open conformation, as seen in the TmTK-TP4A complex structure, is required to provide space for the adenosine moiety. Our analysis supports the formation of an analogous open conformation in hTK1 upon ATP binding.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.