ArticleThe Journal of experimental medicine1992
A nephritogenic rat monoclonal antibody to mouse aminopeptidase A. Induction of massive albuminuria after a single intravenous injection.
Article in The Journal of experimental medicine, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 80 citations in OpenAlex.
- Animal models of idiopathic membranous nephropathy: Recent advances and future perspectives.Animal models and experimental medicine · 2026Review
- Animal models of membranous nephropathy: more choices and higher similarity.Frontiers in immunology · 2024Review
- Bioinformatic characterization of ENPEP, the gene encoding a potential cofactor for SARS-CoV-2 infection.PloS one · 2024Article
- "Idiopathic" minimal change nephrotic syndrome: a podocyte mystery nears the end.American journal of physiology. Renal physiology · 2023Review
- Membranous nephropathy: Clearer pathology and mechanisms identify potential strategies for treatment.Frontiers in immunology · 2022Review
- Advances of the experimental models of idiopathic membranous nephropathy (Review).Molecular medicine reports · 2020Review
- The zinc fingers and homeoboxes 2 protein ZHX2 and its interacting proteins regulate upstream pathways in podocyte diseases.Kidney international · 2020Article
- Deficiency of the Angiotensinase Aminopeptidase A Increases Susceptibility to Glomerular Injury.Journal of the American Society of Nephrology : JASN · 2017Article
- Animal models of nephrotic syndrome.Pediatric nephrology (Berlin, Germany) · 2013Review
- Mouse models of membranous nephropathy: the road less travelled by.American journal of clinical and experimental immunology · 2013Article
- Mutant DLX 3 disrupts odontoblast polarization and dentin formation.Developmental biology · 2010Article
- Autoimmunity in membranous nephropathy targets aldose reductase and SOD2.Journal of the American Society of Nephrology : JASN · 2010Article
- Experimental Models of Membranous Nephropathy.Drug discovery today. Disease models · 2010Article
- Article
- Target antigens and nephritogenic antibodies in membranous nephropathy: of rats and men.Seminars in immunopathology · 2007Review
- Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability.The Journal of clinical investigation · 2003Article
- Loss of glomerular foot processes is associated with uncoupling of podocalyxin from the actin cytoskeleton.The Journal of clinical investigation · 2001Article
- Glomerular overproduction of oxygen radicals in Mpv17 gene-inactivated mice causes podocyte foot process flattening and proteinuria: A model of steroid-resistant nephrosis sensitive to radical scavenger therapy.The American journal of pathology · 1999Article
- Antibody induced injury to podocytes with proteinuria and foot process swelling in a transgenic (T16) mouse.International journal of experimental pathology · 1999Article
- B cells and autoantibodies in the pathogenesis of lupus nephritis.Immunologic research · 1998Review
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Antibodies directed against antigens present on renal epithelial cells can cause membranous glomerulonephritis in experimental animals, which closely resembles the human form of this disease. However, most antibodies produced so far fail to cause the persistent and severe proteinuria that is seen in humans. In our search for new antibodies of this kind, we have now produced a monoclonal antibody (mAb) against mouse aminopeptidase A, a hydrolase that is present in the mouse kidney. The mAb (ASD-4) was prepared by fusion of mouse myeloma cells with splenocytes of Lou rats immunized with brush border (BB) membranes from mouse kidneys. ASD-4 is of the IgG1 subclass and reacts with a 140-kD protein as demonstrated by immunoprecipitation on radiolabeled BB membranes. In indirect immunofluorescence and immunoelectronmicroscopy of normal mouse kidneys, ASD-4 was diffusely present on the BB of the S1 and S2 segments of the proximal tubules, and on the cell membranes of the glomerular visceral epithelia. It also bound to cell membranes of nonglomerular endothelia, smooth muscle cells of arteries, and juxtaglomerular cells. After injection of ASD-4 into normal mice, an immediate homogeneous binding to the capillary wall was seen that gradually changed into a fine granular pattern after 1 d. This glomerular binding was followed by binding to the BB and basolateral membranes of the convoluted proximal tubules. Immediately after injection of ASD-4, a dose-dependent albuminuria occurred that lasted for at least 16 d. ASD-4 is thus a new rat mAb against a well-defined renal epithelial antigen that causes not only membranous glomerulonephritis after a single injection in the mouse, but also severe albuminuria.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.