ArticleNature protocols2006
Genomic systematic evolution of ligands by exponential enrichment (Genomic SELEX) for the identification of protein-binding RNAs independent of their expression levels.
Article in Nature protocols, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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Who cites it
31 citing papers in PubMed, 67 citations in OpenAlex.
- Global Identification of RNA Helicase Substrates from Large Nucleic Acid Sequence Libraries Using Helicase-SELEX.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Guidelines to Study Transcriptome Remodeling by Proteins Using Deep Sequencing (RNA-Seq).Methods in molecular biology (Clifton, N.J.) · 2027Article
- Harnessing deep learning to accelerate the development of antibodies and aptamers.Acta pharmaceutica Sinica. B · 2026Review
- Advances in aptamer-based biosensors for monitoring foodborne pathogens.Journal of food science and technology · 2024Review
- Development of RNA G-quadruplex (rG4)-targeting L-RNA aptamers by rG4-SELEX.Nature protocols · 2022Review
- Recent Advances in Aptasensors For Rapid and Sensitive Detection ofFrontiers in bioengineering and biotechnology · 2022Review
- Recent advances on aptamer-based biosensors for detection of pathogenic bacteria.World journal of microbiology & biotechnology · 2021Review
- Genomic and cDNA selection-amplification identifies transcriptome-wide binding sites for the Drosophila protein sex-lethal.PloS one · 2021Article
- Roles of Emerging RNA-Binding Activity of cGAS in Innate Antiviral Response.Frontiers in immunology · 2021Review
- Implementation of High-Throughput Sequencing (HTS) in Aptamer Selection Technology.International journal of molecular sciences · 2020Review
- RNA polymerase II-binding aptamers in human ACRO1 satellites disrupt transcriptionTranscription · 2020Article
- Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR.Nucleic acids research · 2019Article
- Article
- Multiplex Aptamer Discovery through Apta-Seq and Its Application to ATP Aptamers Derived from Human-Genomic SELEX.ACS chemical biology · 2017Article
- Natural RNA Polymerase Aptamers Regulate Transcription in E. coli.Molecular cell · 2017Article
- Characterization of homing endonuclease binding and cleavage specificities using yeast surface display SELEX (YSD-SELEX).Nucleic acids research · 2017Article
- Acousto-microfluidics for screening of ssDNA aptamer.Scientific reports · 2016Article
- Aptamer-Based Technologies in Foodborne Pathogen Detection.Frontiers in microbiology · 2016Review
- Revealing protein-lncRNA interaction.Briefings in bioinformatics · 2016Review
- Identification of RNA targets for the nuclear multidomain cyclophilin atCyp59 and their effect on PPIase activity.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genomic systematic evolution of ligands by exponential enrichment (Genomic SELEX) is an experimental procedure for the expression condition-independent identification of protein-binding RNAs. RNA libraries derived from genomic DNA are generated via random priming, PCR amplification and in vitro transcription. Libraries consist of genomic sequences of selected size, and fragments are flanked by constant sequences required for amplification and transcription. This RNA pool is then subjected to several rounds of selection and amplification to enrich for RNAs meeting the selection criteria. Various selection criteria are possible. Here we describe selection by affinity to a protein of interest. High-affinity ligands can then be cloned and sequenced to allow their identification. With this method, protein-binding RNAs can be discovered, nucleic acid-protein interactions can be identified, and whole protein-nucleic acid networks can be defined. This method is also suitable for discovering novel genes, including non-protein-coding RNAs, and it complements in silico approaches. It is better suited to detect protein-binding RNAs that are differentially expressed (and therefore absent from many tissues) and low-abundance RNAs than experimental procedures that start from the isolation of expressed RNAs. The protocol takes approximately 3 months to complete.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.