ArticleNature protocols2006
Long-term microfluidic cultures of myotube microarrays for high-throughput focal stimulation.
Article in Nature protocols, 2006. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 52 citations in OpenAlex.
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- Digital Manufacturing of Selective Porous Barriers in Microchannels Using Multi-Material Stereolithography.Micromachines · 2018Article
- Controlling Confinement and Topology to Study Collective Cell Behaviors.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Generation of human muscle fibers and satellite-like cells from human pluripotent stem cells in vitro.Nature protocols · 2016Article
- An open-chamber flow-focusing device for focal stimulation of micropatterned cells.Biomicrofluidics · 2016Article
- Tissue fusion over nonadhering surfaces.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Architecture and migration of an epithelium on a cylindrical wire.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Laminar stream of detergents for subcellular neurite damage in a microfluidic device: a simple tool for the study of neuroregeneration.Journal of neural engineering · 2013Article
- Microfluidic systems for studying neurotransmitters and neurotransmission.Lab on a chip · 2013Review
- Label-free quantitation of peptide release from neurons in a microfluidic device with mass spectrometry imaging.Lab on a chip · 2012Article
- Article
- Direct patterning of coplanar polyethylene glycol alkylsilane monolayers by deep-ultraviolet photolithography as a general method for high fidelity, long-term cell patterning and culture.Journal of vacuum science and technology. B, Nanotechnology & microelectronics : materials, processing, measurement, & phenomena : JVST B · 2011Article
- Growth of primary embryo cells in a microculture system.Biomedical microdevices · 2010Article
- Extensive adipogenic and osteogenic differentiation of patterned human mesenchymal stem cells in a microfluidic device.Lab on a chip · 2010Article
- Soft lithography for micro- and nanoscale patterning.Nature protocols · 2010Article
- Integrated microfluidic devices for combinatorial cell-based assays.Biomedical microdevices · 2009Article
- Screening individual hybridomas by microengraving to discover monoclonal antibodies.Nature protocols · 2009Article
- Localized acetylcholine receptor clustering dynamics in response to microfluidic focal stimulation with agrin.Biophysical journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
We have developed a microfluidic cell culture method that allows for the formation of linear isolated myotubes organized in a parallel microarray. Attachment and spreading of cells are confined within microtracks of cell-adherent proteins separated by a protein-repellent coating. Signaling molecules or other molecules of interest can be focally delivered to the myotubes using heterogeneous microfluidic streams. We have used the method to focally deliver agrin (a molecule implicated as a postsynaptic organizer), which leads to localized acetylcholine receptor clustering. These techniques can be modified to accommodate other cell types and can be adapted to virtually any bioactive molecule such as signaling factors or drugs. This protocol features two major techniques that can be utilized simultaneously or independently to (i) micropattern cells using surface chemical modification and (ii) use a microfluidic platform for culturing and focal stimulation of cells with molecules of interest. Device design, fabrication and assembly can be completed in 3 days.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.