ArticleThe Journal of cell biology1992
Mechanism of centrosome positioning during the wound response in BSC-1 cells.
Article in The Journal of cell biology, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Enucleated cells reveal differential roles of the nucleus in cell migration, polarity, and mechanotransduction.The Journal of cell biology · 2018Article
- HECTD1 controls the protein level of IQGAP1 to regulate the dynamics of adhesive structures.Cell communication and signaling : CCS · 2017Article
- Review
- Centrosome positioning in non-dividing cells.Protoplasma · 2016Review
- An on-chip study on the influence of geometrical confinement and chemical gradient on cell polarity.Biomicrofluidics · 2014Article
- Geometric control of cell migration.Scientific reports · 2013Article
- Ste20-like protein kinase SLK (LOSK) regulates microtubule organization by targeting dynactin to the centrosome.Molecular biology of the cell · 2013Article
- The novel actin/focal adhesion-associated protein MISP is involved in mitotic spindle positioning in human cells.Cell cycle (Georgetown, Tex.) · 2013Article
- Centrosome positioning in vertebrate development.Journal of cell science · 2012Review
- Article
- SMRT analysis of MTOC and nuclear positioning reveals the role of EB1 and LIC1 in single-cell polarization.Journal of cell science · 2011Article
- Orientation and function of the nuclear-centrosomal axis during cell migration.Current opinion in cell biology · 2011Review
- Dynamic instability-driven centering/segregating mechanism in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Rear polarization of the microtubule-organizing center in neointimal smooth muscle cells depends on PKCα, ARPC5, and RHAMM.The American journal of pathology · 2011Article
- Intracellular organelles mediate cytoplasmic pulling force for centrosome centration in the Caenorhabditis elegans early embryo.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Symmetry, stability, and reversibility properties of idealized confined microtubule cytoskeletons.Biophysical journal · 2010Article
- Short-term molecular polarization of cells on symmetric and asymmetric micropatterns.Soft matter · 2010Article
- Golgi polarity does not correlate with speed or persistence of freely migrating fibroblasts.European journal of cell biology · 2009Article
- Adaptive-control model for neutrophil orientation in the direction of chemical gradients.Biophysical journal · 2009Article
- Activation of Galphai3 triggers cell migration via regulation of GIV.The Journal of cell biology · 2008Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Locomoting cells are characterized by a pronounced external and internal anterior-posterior polarity. One of the events associated with cell polarization at the onset of locomotion is a shift of the centrosome, or MTOC, ahead of the nucleus. This position is believed to be of strategic importance for directional cell movement and cell polarity. We have used BSC-1 cells at the edge of an in vitro wound to clarify the causal relationship between MTOC position and the initiation of cell polarization. We find that pronounced cell polarization (the extension of a lamellipod) can take place in the absence of MTOC repositioning or microtubules. Conversely, MTOCs will reposition even after lamellar extension and cell polarization have occurred. Repositioning requires microtubules that extend to the cell periphery and is independent of selective detyrosination of microtubules extending towards the cell front. Significantly, MTOCs maintain, or at least attempt to maintain, a position at the cell's centroid. This is most clearly demonstrated in wounded monolayers of enucleated cells where the MTOC closely follows the centroid position. We suggest that the primary response to the would is the biased extension of a lamellipod, which can occur in the absence of microtubules and MTOC repositioning. Lamellipod extension leads to a shift of the cell's centroid towards the wound. The MTOC, in an attempt to maintain a position near the cell center, will follow. This will automatically put the MTOC ahead of the nucleus in the vast majority of cells. The nucleus as a reference for MTOC position may not be as meaningful as previously thought.
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