ArticleInfection and immunity2007
RNA interference-mediated silencing of the YPS3 gene of Histoplasma capsulatum reveals virulence defects.
Article in Infection and immunity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 66 citations in OpenAlex.
- Hybrid histidine kinases and antifungal warfare in thermal dimorphic fungi.Antimicrobial agents and chemotherapy · 2026Review
- Auranofin is active against Histoplasma capsulatum and reduces the expression of virulence-related genes.PLoS neglected tropical diseases · 2024Article
- Genotypic diversity, virulence, and molecular genetic tools inMicrobiology and molecular biology reviews : MMBR · 2024Review
- Pathogenicity & virulence ofVirulence · 2022Review
- Differential miRNA Expression in Human Macrophage-Like Cells Infected withJournal of fungi (Basel, Switzerland) · 2021Article
- Heat Shock Protein 60, Insights to Its Importance inFrontiers in cellular and infection microbiology · 2020Article
- Turning on virulence: Mechanisms that underpin the morphologic transition and pathogenicity ofVirulence · 2019Review
- Fantastic yeasts and where to find them: the hidden diversity of dimorphic fungal pathogens.Current opinion in microbiology · 2019Review
- Recent admixture between species of the fungal pathogenEvolution letters · 2018Article
- O-Mannosylation of Proteins EnablesmBio · 2018Article
- Nanotechnology-based drug delivery systems for control of microbial biofilms: a review.International journal of nanomedicine · 2018Review
- Article
- Fungal Dimorphism and Virulence: Molecular Mechanisms for Temperature Adaptation, Immune Evasion, and In Vivo Survival.Mediators of inflammation · 2017Review
- The Mould-specific M46 gene is not essential for yeast-mould dimorphism in the pathogenic fungus Histoplasma capsulatum.Medical mycology · 2016Article
- Revisiting old friends: Developments in understanding Histoplasma capsulatum pathogenesis.Journal of microbiology (Seoul, Korea) · 2016Review
- Histoplasma capsulatum surmounts obstacles to intracellular pathogenesis.The FEBS journal · 2016Review
- Article
- Opportunistic yeast pathogens: reservoirs, virulence mechanisms, and therapeutic strategies.Cellular and molecular life sciences : CMLS · 2015Review
- Dimorphism in fungal pathogens of mammals, plants, and insects.PLoS pathogens · 2015Review
- Genome update of the dimorphic human pathogenic fungi causing paracoccidioidomycosis.PLoS neglected tropical diseases · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The YPS3 gene of Histoplasma capsulatum encodes a protein that is both surface localized in the cell wall of H. capsulatum and released into the culture medium. This protein is produced only during the pathogenic yeast phase of infection and is also expressed differentially in H. capsulatum strains of different virulence levels. In this study, we silenced the YPS3 transcript by using an interfering-RNA strategy and examined the silenced mutants for phenotypic differences in vitro and during infection. The mutants showed no growth defect during in vitro culture in a defined medium at 37 degrees C and appeared to have normal virulence in a RAW 264.7 murine macrophage-like cell line. In a C57BL/6 mouse model of infection, however, the mutants caused significantly decreased fungal burdens, particularly in the peripheral phagocyte-rich tissues of livers and spleens. This defect in organ colonization was evident within 3 days of infection; however, it appeared to be exacerbated at later time points.
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