ArticleNucleic acids research2007
Bidirectional transcription is an inherent feature of Giardia lamblia promoters and contributes to an abundance of sterile antisense transcripts throughout the genome.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
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- Long Non-Coding RNAs in the Regulation of Gene Expression: Physiology and Disease.Non-coding RNA · 2019Review
- Differential protein expression and post-translational modifications in metronidazole-resistant Giardia duodenalis.GigaScience · 2018Article
- ChIP-ping the branches of the tree: functional genomics and the evolution of eukaryotic gene regulation.Briefings in functional genomics · 2018Review
- Time-Dependent Transcriptional Changes in Axenic Giardia duodenalis Trophozoites.PLoS neglected tropical diseases · 2015Article
- The genome of Spironucleus salmonicida highlights a fish pathogen adapted to fluctuating environments.PLoS genetics · 2014Article
- Small RNA pyrosequencing in the protozoan parasite Entamoeba histolytica reveals strain-specific small RNAs that target virulence genes.BMC genomics · 2013Article
- Transcriptome profiling of Giardia intestinalis using strand-specific RNA-seq.PLoS computational biology · 2013Article
- Article
- Identification and analysis of the RNA degrading complexes and machinery of Giardia lamblia using an in silico approach.BMC genomics · 2011Article
- Characterization of RNase MRP RNA and novel snoRNAs from Giardia intestinalis and Trichomonas vaginalis.BMC genomics · 2011Article
- Characterizing ncRNAs in Human Pathogenic Protists Using High-Throughput Sequencing Technology.Frontiers in genetics · 2011Article
- Transcriptome analyses of the Giardia lamblia life cycle.Molecular and biochemical parasitology · 2010Article
- Identification of Scaffold/Matrix Attachment (S/MAR) like DNA element from the gastrointestinal protozoan parasite Giardia lamblia.BMC genomics · 2010Article
- Mouse Dfa is a repressor of TATA-box promoters and interacts with the Abt1 activator of basal transcription.The Journal of biological chemistry · 2010Article
- Identification of transcriptional signals in Encephalitozoon cuniculi widespread among Microsporidia phylum: support for accurate structural genome annotation.BMC genomics · 2009Article
- Article
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3 authors at 1 institution in 1 country.
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Abstract
A prominent feature of transcription in Giardia lamblia is the abundant production of sterile antisense transcripts (Elmendorf et al. The abundance of sterile transcripts in Giardia lamblia. Nucleic Acids., 29, 4674-4683). Here, we use a computational biology analysis of SAGE data to assess the abundance and distribution of sense and antisense messages in the parasite genome. Sterile antisense transcripts are produced at approximately 50% of loci with detectable transcription, yet their abundance at a given locus does not correlate to the abundance of the complementary sense transcripts at that locus or to transcription levels at neighboring loci. These data suggest that sterile antisense transcripts are not simply a local effect of open chromatin structure. Using 5'RACE, we demonstrate that Giardia promoters are a source of antisense transcripts through bidirectional transcription, producing both downstream coding sense and upstream sterile antisense transcripts. We use a dual reporter system to explore roles of specific promoter elements in this bidirectional initiation of transcription and suggest that the degenerate AT-rich nature of TATA and Inr elements in Giardia permits them to function interchangeably. The phenomenon of bidirectional transcription in G. lamblia gives us insight into the interaction between transcriptional machinery and promoter elements, and may be the prominent source of the abundant antisense transcription in this parasite.
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