ArticleJournal of neuroscience methods2007
Pepsin pretreatment allows collagen IV immunostaining of blood vessels in adult mouse brain.
Article in Journal of neuroscience methods, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Intramural hematomas and astrocytic infiltration precede perivascular inflammation in a rat model of repetitive low-level blast injury.Journal of neuropathology and experimental neurology · 2025Article
- Creation of a novel CRISPR-generated allele to express HA epitope-tagged C1QL1 and improved methods for its detection at synapses.FEBS letters · 2024Article
- Using digital pathology to analyze the murine cerebrovasculature.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2024Article
- The Neurovascular Unit as a Locus of Injury in Low-Level Blast-Induced Neurotrauma.International journal of molecular sciences · 2024Review
- Late chronic local inflammation, synaptic alterations, vascular remodeling and arteriovenous malformations in the brains of male rats exposed to repetitive low-level blast overpressures.Acta neuropathologica communications · 2023Article
- Glyoxal Fixation Is Optimal for Immunostaining of Brain Vessels, Pericytes and Blood-Brain Barrier Proteins.International journal of molecular sciences · 2022Article
- Article
- Low-level blast exposure induces chronic vascular remodeling, perivascular astrocytic degeneration and vascular-associated neuroinflammation.Acta neuropathologica communications · 2021Article
- Obesity-associated hyperleptinemia alters the gliovascular interface of the hypothalamus to promote hypertension.Cell metabolism · 2021Article
- Exploring How Low Oxygen Post Conditioning Improves Stroke-Induced Cognitive Impairment: A Consideration of Amyloid-Beta Loading and Other Mechanisms.Frontiers in neurology · 2021Article
- Organisation of extracellular matrix proteins laminin and agrin in pericapillary basal laminae in mouse brain.Brain structure & function · 2020Article
- Exercise prevents obesity-induced cognitive decline and white matter damage in mice.Neurobiology of aging · 2019Article
- Arterial Stiffness Due to Carotid Calcification Disrupts Cerebral Blood Flow Regulation and Leads to Cognitive Deficits.Journal of the American Heart Association · 2019Article
- Low-level blast exposure disrupts gliovascular and neurovascular connections and induces a chronic vascular pathology in rat brain.Acta neuropathologica communications · 2019Article
- Chronic stress induced disruption of the peri-infarct neurovascular unit following experimentally induced photothrombotic stroke.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2017Article
- A novel fluorescent imaging technique for assessment of cerebral vasospasm after experimental subarachnoid hemorrhage.Scientific reports · 2017Article
- Lack of functional P2X7 receptor aggravates brain edema development after middle cerebral artery occlusion.Purinergic signalling · 2016Article
- Formaldehyde scavengers function as novel antigen retrieval agents.Scientific reports · 2015Article
- APOE Stabilization by Exercise Prevents Aging Neurovascular Dysfunction and Complement Induction.PLoS biology · 2015Article
- Vascular and inflammatory factors in the pathophysiology of blast-induced brain injury.Frontiers in neurology · 2015Review
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
Abstract
While the brain vasculature can be imaged with many methods, immunohistochemistry has distinct advantages due to its simplicity and applicability to archival tissue. However, immunohistochemical staining of the murine brain vasculature in aldehyde fixed tissue has proven elusive and inconsistent using current protocols. Here we investigated whether antigen retrieval methods could improve vascular staining in the adult mouse brain. We found that pepsin digestion prior to immunostaining unmasked widespread collagen IV staining of the cerebrovasculature in the adult mouse brain. Pepsin treatment also unmasked widespread vascular staining with laminin, but only marginally improved isolectin B4 staining and did not enhance vascular staining with fibronectin, perlecan or CD146. Collagen IV immunoperoxidase staining was easily combined with cresyl violet counterstaining making it suitable for stereological analyses of both vascular and neuronal parameters in the same tissue section. This method should be widely applicable for labeling the brain vasculature of the mouse in aldehyde fixed tissue from both normal and pathological states.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.