ArticleHuman genetics1992
Rapid generation of chromosome-specific alphoid DNA probes using the polymerase chain reaction.
Article in Human genetics, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 60 citations in OpenAlex.
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- In-vivo fusion of human cancer and hamster stromal cells permanently transduces and transcribes human DNA.PloS one · 2014Article
- Persistence of DNA threads in human anaphase cells suggests late completion of sister chromatid decatenation.Chromosoma · 2008Article
- Sequence analysis of a functional Drosophila centromere.Genome research · 2003Article
- Non-random radial higher-order chromatin arrangements in nuclei of diploid human cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2001Article
- Isolation of DNA from the centromere of human chromosome 7 by microdissection.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 1997Article
- Active and inactive genes localize preferentially in the periphery of chromosome territories.The Journal of cell biology · 1996Article
- Detection of aneuploidy in human spermatozoa of normal semen donors by fluorescence in situ hybridization.Environmental health perspectives · 1996Article
- Article
- PCR amplification of tandemly repeated DNA: analysis of intra- and interchromosomal sequence variation and homologous unequal crossing-over in human alpha satellite DNA.Nucleic acids research · 1992Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Non-isotopic in situ hybridization of chromosome-specific alphoid DNA probes has become a potent tool in the study of numerical aberrations of specific human chromosomes at all stages of the cell cycle. In this paper, we describe approaches for the rapid generation of such probes using the polymerase chain reaction (PCR), and demonstrate their chromosome specificity by fluorescence in situ hybridization to normal human metaphase spreads and interphase nuclei. Oligonucleotide primers for conserved regions of the alpha satellite monomer were used to generate chromosome-specific DNA probes from somatic hybrid cells containing various human chromosomes, and from DNA libraries from sorted human chromosomes. Oligonucleotide primers for chromosome-specific regions of the alpha satellite monomer were used to generate specific DNA probes for the pericentromeric heterochromatin of human chromosomes 1, 6, 7, 17 and X directly from human genomic DNA.
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