ArticleNeurochemical research2007
Endogenous expression of adenosine A1, A2 and A3 receptors in rat C6 glioma cells.
Article in Neurochemical research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Antitumoral Action of Resveratrol Through Adenosinergic Signaling in C6 Glioma Cells.Frontiers in neuroscience · 2021Article
- Modulation of Adenosine Receptors and Antioxidative Effect of Beer Extracts in in Vitro Models.Nutrients · 2019Article
- Membrane cholesterol access into a G-protein-coupled receptor.Nature communications · 2017Article
- Binding and Signaling Studies Disclose a Potential Allosteric Site for Cannabidiol in Cannabinoid CBFrontiers in pharmacology · 2017Article
- A genomics approach identifies selective effects of trans-resveratrol in cerebral cortex neuron and glia gene expression.PloS one · 2017Article
- Adenosine A3 receptor elicits chemoresistance mediated by multiple resistance-associated protein-1 in human glioblastoma stem-like cells.Oncotarget · 2016Article
- Tricyclic Antidepressant Amitriptyline-induced Glial Cell Line-derived Neurotrophic Factor Production Involves Pertussis Toxin-sensitive Gαi/o Activation in Astroglial Cells.The Journal of biological chemistry · 2015Article
- Modulation of A1 and A2B adenosine receptor activity: a new strategy to sensitise glioblastoma stem cells to chemotherapy.Cell death & disease · 2014Article
- Differential expression and role of hyperglycemia induced oxidative stress in epigenetic regulation of β1, β2 and β3-adrenergic receptors in retinal endothelial cells.BMC medical genomics · 2014Article
- Purinergic signalling and cancer.Purinergic signalling · 2013Review
- Adenosine inhibits renin release from juxtaglomerular cells via an A1 receptor-TRPC-mediated pathway.American journal of physiology. Renal physiology · 2013Article
- Involvement of ecto-5'-nucleotidase/CD73 in U138MG glioma cell adhesion.Molecular and cellular biochemistry · 2012Article
- Modulation of adenosine receptors by [60]fullerene hydrosoluble derivative in SK-N-MC cells.ACS chemical neuroscience · 2011Article
- Glutamate differently modulates metabotropic glutamate receptors in neuronal and glial cells.Neurochemical research · 2010Article
- Signalling and pharmacological properties of the P2Y receptor.Acta physiologica (Oxford, England) · 2010Review
- Stimulation of the adenosine A3 receptor reverses vascular hyporeactivity after hemorrhagic shock in rats.Acta pharmacologica Sinica · 2010Article
- Glioblastoma cells express functional cell membrane receptors activated by daily used medical drugs.Journal of cancer research and clinical oncology · 2009Article
- Gi-dependent cell signaling responses of the human P2Y14 receptor in model cell systems.The Journal of pharmacology and experimental therapeutics · 2009Article
- Species comparison of adenosine receptor subtypes in brain and testis.Neurochemical research · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inhibitory and stimulatory adenosine receptors have been identified and characterized in both membranes and intact rat C6 glioma cells. In membranes, saturation experiment performed with [(3)H]DPCPX, selective A(1)R antagonist, revealed a single binding site with a K (D) = 9.4 +/- 1.4 nM and B (max) = 62.7 +/- 8.6 fmol/mg protein. Binding of [(3)H]DPCPX in intact cell revealed a K (D) = 17.7 +/- 1.3 nM and B (max )= 567.1 +/- 26.5 fmol/mg protein. On the other hand, [(3)H]ZM241385 binding experiments revealed a single binding site population of receptors with K (D) = 16.5 +/- 1.3 nM and B (max) = 358.9 +/- 52.4 fmol/mg protein in intact cells, and K (D) = 4.7 +/- 0.6 nM and B (max) = 74.3 +/- 7.9 fmol/mg protein in plasma membranes, suggesting the presence of A(2A) receptor in C6 cells. A(1), A(2A), A(2B) and A(3 )adenosine receptors were detected by Western-blotting and immunocytochemistry, and their mRNAs quantified by real time PCR assays. Gialpha and Gsalpha proteins were also detected by Western-blotting and RT-PCR assays. Furthermore, selective A(1)R agonists inhibited forskolin- and GTP-stimulated adenylyl cyclase activity and CGS 21680 and NECA stimulated this enzymatic activity in C6 cells. These results suggest that C6 glioma cells endogenously express A(1) and A(2) receptors functionally coupled to adenylyl cyclase inhibition and stimulation, respectively, and suggest these cells as a model to study the role of adenosine receptors in tumoral cells.
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