ArticleProtein science : a publication of the Protein Society2007
Ligand binding and allostery can emerge simultaneously.
Article in Protein science : a publication of the Protein Society, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 35 citations in OpenAlex.
- Converting a Periplasmic Binding Protein into a Synthetic Biosensing Switch through Domain Insertion.BioMed research international · 2019Review
- Incorporating an allosteric regulatory site in an antibody through backbone design.Protein science : a publication of the Protein Society · 2017Article
- A combination of mutational and computational scanning guides the design of an artificial ligand-binding controlled lipase.Scientific reports · 2017Article
- The Promiscuity of Allosteric Regulation of Nuclear Receptors by Retinoid X Receptor.The journal of physical chemistry. B · 2016Article
- Evolution of a G protein-coupled receptor response by mutations in regulatory network interactions.Nature communications · 2016Article
- Rational design of a fusion protein to exhibit disulfide-mediated logic gate behavior.ACS synthetic biology · 2015Article
- Article
- Serine protease activity of calnuc: regulation by Zn2+ and G proteins.The Journal of biological chemistry · 2013Article
- Protein switch engineering by domain insertion.Methods in enzymology · 2013Article
- Protein conformational switches: from nature to design.Chemistry (Weinheim an der Bergstrasse, Germany) · 2012Review
- Structure of an engineered β-lactamase maltose binding protein fusion protein: insights into heterotropic allosteric regulation.PloS one · 2012Article
- A genetically encoded, high-signal-to-noise maltose sensor.Proteins · 2011Article
- Converting a protein into a switch for biosensing and functional regulation.Protein science : a publication of the Protein Society · 2011Review
- Designing switchable enzymes.Current opinion in structural biology · 2009Review
- Generation of new protein functions by nonhomologous combinations and rearrangements of domains and modules.Current opinion in biotechnology · 2009Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A heterotropic allosteric effect involves an effector molecule that is distinct from the substrate or ligand of the protein. How heterotropic allostery originates is an unanswered question. We have previously created several heterotropic allosteric enzymes by recombining the genes for TEM1 beta-lactamase (BLA) and maltose binding protein (MBP) to create BLAs that are positively or negatively regulated by maltose. We show here that one of these engineered enzymes has approximately 10(6) M(-1) affinity for Zn(2+), a property that neither of the parental proteins possesses. Furthermore, Zn(2+) is a negative effector that noncompetitively switches off beta-lactam hydrolysis activity. Mutagenesis experiments indicate that the Zn(2+)-binding site does not involve a histidine or a cysteine, which is atypical of natural Zn(2+)-binding sites. These studies also implicate helices 1 and 12 of the BLA domain in allosteric signal propagation. These results support a model for the evolution of heterotropic allostery in which effector affinity and allosteric signaling emerge simultaneously.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.