ArticleThe Plant cell2007
Arabidopsis phage-type RNA polymerases: accurate in vitro transcription of organellar genes.
Article in The Plant cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Professor Thomas Börner: the pioneer of molecular plant genetics in East Germany turns 80!Molecular genetics and genomics : MGG · 2026Article
- Artificial Mutations in the Nuclear Gene Encoding Mitochondrial RNA Polymerase Restore Pollen Fertility in Cytoplasmic Male Sterile Tomato.Plant biotechnology journal · 2026Article
- Membranous Interacting Partners of Phage-Type Plastid RNA Polymerase Have Limited Impact on Plastid Gene Expression During Chloroplast Development.Plant direct · 2026Article
- RNA Polymerase RPOTp is Involved in C-to-U RNA Editing at Multiple Sites in Arabidopsis Chloroplasts.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- NNature communications · 2022Article
- A Transcription Factor Regulates Gene Expression in Chloroplasts.International journal of molecular sciences · 2021Article
- Identification, Characterization, and Expression Profile Analysis of theFrontiers in plant science · 2021Article
- Complete Sequence, Multichromosomal Architecture and Transcriptome Analysis of theInternational journal of molecular sciences · 2019Article
- CyMIRA: The Cytonuclear Molecular Interactions Reference for Arabidopsis.Genome biology and evolution · 2019Article
- Control of organelle gene expression by the mitochondrial transcription termination factor mTERF22 in Arabidopsis thaliana plants.PloS one · 2018Article
- Analysis of the Roles of the Arabidopsis nMAT2 and PMH2 Proteins Provided with New Insights into the Regulation of Group II Intron Splicing in Land-Plant Mitochondria.International journal of molecular sciences · 2017Article
- Highly Conserved Elements and Chromosome Structure Evolution in Mitochondrial Genomes in Ciliates.Life (Basel, Switzerland) · 2017Article
- The [4Fe-4S] clusters of Rpo3 are key determinants in the post Rpo3/Rpo11 heterodimer formation of RNA polymerase in Methanosarcina acetivorans.MicrobiologyOpen · 2017Article
- In vitro promoter recognition by the catalytic subunit of plant phage-type RNA polymerases.Plant molecular biology · 2016Article
- RHON1 mediates a Rho-like activity for transcription termination in plastids of Arabidopsis thaliana.The Plant cell · 2014Article
- Enzymes involved in organellar DNA replication in photosynthetic eukaryotes.Frontiers in plant science · 2014Review
- Localization and phylogenetic analysis of enzymes related to organellar genome replication in the unicellular rhodophyte Cyanidioschyzon merolae.Genome biology and evolution · 2014Article
- Genome-wide identification, evolution and expression analysis of mTERF gene family in maize.PloS one · 2014Article
- The genome and transcriptome of perennial ryegrass mitochondria.BMC genomics · 2013Article
- Expression of plastid genes: organelle-specific elaborations on a prokaryotic scaffold.Plant physiology · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The T7 bacteriophage RNA polymerase (RNAP) performs all steps of transcription, including promoter recognition, initiation, and elongation as a single-polypeptide enzyme. Arabidopsis thaliana possesses three nuclear-encoded T7 phage-type RNAPs that localize to mitochondria (RpoTm), plastids (RpoTp), or presumably both organelles (RpoTmp). Their specific functions are as yet unresolved. We have established an in vitro transcription system to examine the abilities of the three Arabidopsis phage-type RNAPs to synthesize RNA and to recognize organellar promoters. All three RpoT genes were shown to encode transcriptionally active RNAPs. RpoTmp displayed no significant promoter specificity, whereas RpoTm and RpoTp were able to accurately initiate transcription from overlapping subsets of mitochondrial and plastidial promoters without the aid of protein cofactors. Our study strongly suggests RpoTm to be the enzyme that transcribes most, if not all, mitochondrial genes in Arabidopsis. Intrinsic promoter specificity, a feature that RpoTm and RpoTp share with the T7 RNAP, appears to have been conserved over the long period of evolution of nuclear-encoded mitochondrial and plastidial RNAPs. Selective promoter recognition by the Arabidopsis phage-type RNAPs in vitro implies that auxiliary factors are required for efficient initiation of transcription in vivo.
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