ArticleApplied and environmental microbiology2007
Identification of unconventional intestinal pathogenic Escherichia coli isolates expressing intermediate virulence factor profiles by using a novel single-step multiplex PCR.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers, 1 of them a synthesis that pooled it.
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Who cites it
113 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Enteroaggregative Escherichia coli and acute diarrhea in children: a meta-analysis of South Asian populations.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2013Pooled it
- Virulence factors associated with pediatric shigellosis in Brazilian Amazon.BioMed research international · 2014Trial
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- Genomic and Pathogenic Characterization of a Novel Capsule-Deficient Neonatal Meningitis-AssociatedVeterinary sciences · 2026Article
- Imported Retail Beef and Chicken Meat Products Serve as Reservoirs for Emerging Antibiotic-Resistant Pathotypes of Escherichia coli in Pristine Areas Free From Agricultural Activity.MicrobiologyOpen · 2026Article
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- Whole-Genome Sequence Analysis of Shiga Toxin-ProducingMicroorganisms · 2026Article
- The Missing Process Hygiene Criteria in the Game Meat Chain: Challenges in Wild Boar Meat Production.Veterinary medicine international · 2026Article
- Occurrence of viral and bacterial pathogens in South American camelids in representative German flocks.Frontiers in veterinary science · 2026Article
- An Optimized PCR Assay to DetectFood safety (Tokyo, Japan) · 2025Article
- Comparative analysis of virulence genes, biofilm production, and antibiotic resistance in Escherichia coli from dogs and humans using rep-PCR.BMC veterinary research · 2025Article
- Antibacterial and Antibiofilm Activity of Green-Synthesized Zinc Oxide Nanoparticles Against Multidrug-ResistantMolecules (Basel, Switzerland) · 2025Article
- Association ofFrontiers in cellular and infection microbiology · 2025Article
- In Vitro Antibacterial Activity of Essential Oils fromPathogens (Basel, Switzerland) · 2024Article
- Antimicrobial Resistance and Pathotypes ofAnimals : an open access journal from MDPI · 2024Article
- An outbreak associated withEpidemiology and infection · 2024Article
- Bacteriophage defends murine gut from Escherichia coli invasion via mucosal adherence.Nature communications · 2024Article
- Escherichia coli and their potential transmission of carbapenem and colistin-resistant genes in camels.BMC microbiology · 2024Article
53 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intestinal pathogenic Escherichia coli represents a global health problem for mammals, including humans. At present, diarrheagenic E. coli bacteria are grouped into seven major pathotypes that differ in their virulence factor profiles, severity of clinical manifestations, and prognosis. In this study, we developed and evaluated a one-step multiplex PCR (MPCR) for the straightforward differential identification of intestinal pathotypes of E. coli. The specificity of this novel MPCR was validated by using a subset of reference strains and further confirmed by PCR-independent pheno- and genotypic characterization. Moreover, we tested 246 clinical E. coli isolates derived from diarrhea patients from several distinct geographic regions. Interestingly, besides strains belonging to the defined and well-described pathotypes, we identified five unconventional strains expressing intermediate virulence factor profiles. These strains have been further characterized and appear to represent intermediate strains carrying genes and expressing factors associated with enteropathogenic E. coli, Shiga toxin-producing E. coli, enterotoxigenic E. coli, and enteroaggregative E. coli alike. These strains represent further examples of the extraordinary plasticity of the E. coli genome. Moreover, this implies that the important identification of specific pathotypes has to be based on a broad matrix of indicator genes. In addition, the presence of intermediate strains needs to be accounted for.
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