ArticleApplied and environmental microbiology2007
Diversity and abundance of nitrate reductase genes (narG and napA), nitrite reductase genes (nirS and nrfA), and their transcripts in estuarine sediments.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 80 papers.
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Who cites it
80 citing papers in PubMed, 315 citations in OpenAlex.
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- Land use changes alter microbial functional gene diversity and its relationship with soil ecosystem multifunctionality in a subtropical estuary.Frontiers in microbiology · 2025Article
- The effect of crop-rotation systems in regenerative agricultural environments on the rhizosphere microbiome of dry-land winter wheat.Sustainable microbiology · 2025Article
- Factors influencing the spatial distributions of river microbial communities at the watershed scale: a case study involving the Wuding River Basin.Frontiers in microbiology · 2025Article
- Magnesium application partially reversed the negative effects of mulching on rhizosphere nitrogen cycling in aFrontiers in plant science · 2025Article
- Hyperexpansion of genetic diversity and metabolic capacity of extremophilic bacteria and archaea in ancient Andean lake sediments.Microbiome · 2024Article
- A seasonal study on the microbiomes of Diploid vs. Triploid eastern oysters and their denitrification potential.iScience · 2024Article
- Denitrification in hypersaline and coastal environments.FEMS microbiology letters · 2023Article
- Effects of different soil water holding capacities on vegetable residue return and its microbiological mechanism.Frontiers in microbiology · 2023Article
- Nitrogen cycling activities during decreased stratification in the coastal oxygen minimum zone off Namibia.Frontiers in microbiology · 2023Article
- Corpse decay of wild animals leads to the divergent succession of nrfA-type microbial communities.Applied microbiology and biotechnology · 2022Article
- The active functional microbes contribute differently to soil nitrification and denitrification potential under long-term fertilizer regimes in North-East China.Frontiers in microbiology · 2022Article
- Dissimilatory nitrate reduction and functional genes in two subtropical rivers, China.Environmental science and pollution research international · 2021Article
- Response of CoastalFrontiers in microbiology · 2021Article
- DiazotrophicApplied and environmental microbiology · 2020Article
- A novel real-world ecotoxicological dataset of pelagic microbial community responses to wastewater.Scientific data · 2020Article
- Article
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20 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Estuarine systems are the major conduits for the transfer of nitrate from agricultural and other terrestrial-anthropogenic sources into marine ecosystems. Within estuarine sediments some microbially driven processes (denitrification and anammox) result in the net removal of nitrogen from the environment, while others (dissimilatory nitrate reduction to ammonium) do not. In this study, molecular approaches have been used to investigate the diversity, abundance, and activity of the nitrate-reducing communities in sediments from the hypernutrified Colne estuary, United Kingdom, via analysis of nitrate and nitrite reductase genes and transcripts. Sequence analysis of cloned PCR-amplified narG, napA, and nrfA gene sequences showed the indigenous nitrate-reducing communities to be both phylogenetically diverse and also divergent from previously characterized nitrate reduction sequences in soils and offshore marine sediments and from cultured nitrate reducers. In both the narG and nrfA libraries, the majority of clones (48% and 50%, respectively) were related to corresponding sequences from delta-proteobacteria. A suite of quantitative PCR primers and TaqMan probes was then developed to quantify phylotype-specific nitrate (narG and napA) and nitrite reductase (nirS and nrfA) gene and transcript numbers in sediments from three sites along the estuarine nitrate gradient. In general, both nitrate and nitrite reductase gene copy numbers were found to decline significantly (P < 0.05) from the estuary head towards the estuary mouth. The development and application, for the first time, of quantitative reverse transcription-PCR assays to quantify mRNA sequences in sediments revealed that transcript numbers for three of the five phylotypes quantified were greatest at the estuary head.
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