ArticleBiophysical journal2007
A new approach to the detection and statistical classification of Ca2+ sparks.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed.
- Article
- Glucose and NAADP trigger elementary intracellular β-cell CaScientific reports · 2021Article
- PunctaSpecks: A tool for automated detection, tracking, and analysis of multiple types of fluorescently labeled biomolecules.Cell calcium · 2020Article
- High Time Resolution Analysis of Voltage-Dependent and Voltage-Independent Calcium Sparks in Frog Skeletal Muscle Fibers.Frontiers in physiology · 2020Article
- CellSpecks: A Software for Automated Detection and Analysis of Calcium Channels in Live Cells.Biophysical journal · 2018Article
- Beta1-adrenoceptor antagonist, metoprolol attenuates cardiac myocyte CaJournal of molecular and cellular cardiology · 2018Article
- Size Matters: Ryanodine Receptor Cluster Size Affects Arrhythmogenic Sarcoplasmic Reticulum Calcium Release.Journal of the American Heart Association · 2018Article
- Impaired BKScientific reports · 2017Article
- Stabilization of diastolic calcium signal via calcium pump regulation of complex local calcium releases and transient decay in a computational model of cardiac pacemaker cell with individual release channels.PLoS computational biology · 2017Article
- Automated detection and analysis of depolarization events in human cardiomyocytes using MaDEC.Computers in biology and medicine · 2016Article
- Fast kinetics of calcium signaling and sensor design.Current opinion in chemical biology · 2015Review
- An algorithm for automated detection, localization and measurement of local calcium signals from camera-based imaging.Cell calcium · 2014Article
- Mechanochemotransduction during cardiomyocyte contraction is mediated by localized nitric oxide signaling.Science signaling · 2014Article
- Automated detection and analysis of Ca(2+) sparks in x-y image stacks using a thresholding algorithm implemented within the open-source image analysis platform ImageJ.Biophysical journal · 2014Article
- Ca²⁺ waves in the heart.Journal of molecular and cellular cardiology · 2013Review
- Isoproterenol increases the fraction of spark-dependent RyR-mediated leak in ventricular myocytes.Biophysical journal · 2013Article
- Tracking stars: automated two-dimensional analysis of Ca²⁺ events. Focus on "Automated region of interest analysis of dynamic Ca²⁺ signals in image sequences".American journal of physiology. Cell physiology · 2012Article
- Superoxide flashes reveal novel properties of mitochondrial reactive oxygen species excitability in cardiomyocytes.Biophysical journal · 2012Article
- Dynamic calcium movement inside cardiac sarcoplasmic reticulum during release.Circulation research · 2011Article
- Single ryanodine receptor channel basis of caffeine's action on Ca2+ sparks.Biophysical journal · 2011Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The availability of high-speed, two-dimensional (2-D) confocal microscopes and the expanding armamentarium of fluorescent probes presents unprecedented opportunities and new challenges for studying the spatial and temporal dynamics of cellular processes. The need to remove subjectivity from the detection process, the difficulty of the human eye to detect subtle changes in fluorescence in these 2-D images, and the large volume of data produced by these confocal microscopes call for the need to develop algorithms to automatically mark the changes in fluorescence. These fluorescence signal changes are often subtle, so the statistical estimate of the likelihood that the detected signal is not noise is an integral part of the detection algorithm. This statistical estimation is fundamental to our new approach to detection; in earlier Ca(2+) spark detectors, this statistical assessment was incidental to detection. Importantly, the use of the statistical properties of the signal local to the spark, instead of over the whole image, reduces the false positive and false negative rates. We developed an automatic spark detection algorithm based on these principles and used it to detect sparks on an inhomogeneous background of transverse tubule-labeled rat ventricular cells. Because of the large region of the cell surveyed by the confocal microscope, we can detect a large enough number of sparks to measure the dynamic changes in spark frequency in individual cells. We also found, in contrast to earlier results, that cardiac sparks are spatially symmetric. This new approach puts the detection of fluorescent signals on a firm statistical foundation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.