ArticleBiophysical journal2007
Atomic force microscope studies of the fusion of floating lipid bilayers.
Article in Biophysical journal, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 42 citations in OpenAlex.
- DNA Flipping as Facile Mechanism for Transmembrane Signaling in Synthetic Cells.Journal of the American Chemical Society · 2025Article
- Molecular clutch drives cell response to surface viscosity.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Review
- Reconstitution of Human Ion Channels into Solvent-free Lipid Bilayers Enhanced by Centrifugal Forces.Biophysical journal · 2016Article
- Direct observation of intermediate states in model membrane fusion.Scientific reports · 2016Article
- Custom AFM for X-ray beamlines: in situ biological investigations under physiological conditions.Journal of synchrotron radiation · 2015Article
- Penetration of cell membranes and synthetic lipid bilayers by nanoprobes.Biophysical journal · 2014Article
- Preparation of DOPC and DPPC Supported Planar Lipid Bilayers for Atomic Force Microscopy and Atomic Force Spectroscopy.International journal of molecular sciences · 2013Article
- Adhesion energy can regulate vesicle fusion and stabilize partially fused states.Journal of the Royal Society, Interface · 2012Article
- Dynamic force spectroscopy on supported lipid bilayers: effect of temperature and sample preparation.Biophysical journal · 2012Article
- Model system for cell adhesion mediated by weak carbohydrate-carbohydrate interactions.Journal of the American Chemical Society · 2012Article
- Cholesterol-dependent nanomechanical stability of phase-segregated multicomponent lipid bilayers.Biophysical journal · 2010Article
- Examining the origins of the hydration force between lipid bilayers using all-atom simulations.The Journal of membrane biology · 2010Article
- Investigation of SNARE-Mediated Membrane Fusion Mechanism Using Atomic Force Microscopy.Japanese journal of applied physics (2008) · 2009Article
- Pulling force generated by interacting SNAREs facilitates membrane hemifusion.Integrative biology : quantitative biosciences from nano to macro · 2009Article
- Atomic force microscope spectroscopy reveals a hemifusion intermediate during soluble N-ethylmaleimide-sensitive factor-attachment protein receptors-mediated membrane fusion.Biophysical journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
This study investigated the fusion of apposing floating bilayers of egg L-alpha-phosphatidylcholine (egg PC) or 1,2-dimyristoyl-sn-glycero-3-phosphocholine. Atomic force microscope measurements of fusion forces under different compression rates were acquired to reveal the energy landscape of the fusion process under varied lipid composition and temperature. Between compression rates of approximately 1000 and approximately 100,000 pN/s, applied forces in the range from approximately 100 to approximately 500 pN resulted in fusion of floating bilayers. Our atomic force microscope measurements indicated that one main energy barrier dominated the fusion process. The acquired dynamic force spectra were fit with a simple model based on the transition state theory with the assumption that the fusion activation potential is linear. A significant shift in the energy landscape was observed when bilayer fluidity and composition were modified, respectively, by temperature and different cholesterol concentrations (15% < or = chol < or = 25%). Such modifications resulted in a more than twofold increase in the width of the fusion energy barrier for egg PC and 1,2-dimyristoyl-sn-glycero-3-phosphocholine floating bilayers. The addition of 25% cholesterol to egg PC bilayers increased the activation energy by approximately 1.0 k(B)T compared with that of bilayers with egg PC alone. These results reveal that widening of the energy barrier and consequently reduction in its slope facilitated membrane fusion.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.