ArticleExperimental eye research2007
Assay of mucins in human tear fluid.
Article in Experimental eye research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
77 citing papers in PubMed, 1 synthesis or guideline pooled it, 165 citations in OpenAlex.
- Aqueous-deficient dry eye disease: Preferred practice pattern guidelines on clinical approach, diagnosis, and management.Indian journal of ophthalmology · 2023Guideline
- Mucoadhesive nanofibers for ocular drug delivery: mechanisms, design strategies, and applications.Drug delivery and translational research · 2026Review
- Managing dry eye disease - a review of selected traditional Chinese medicine and some of their metabolites focusing on molecular mechanisms and signaling pathways.Frontiers in pharmacology · 2026Review
- MUC1‑ND interacts with TRPV1 to promote corneal epithelial cell proliferation in diabetic dry eye mice by partly activating the AKT signaling pathway.Molecular medicine reports · 2024Article
- Regional Conjunctival Differences in Glycocalyx Mucin Expression in Dry Eye and Normal Subjects.Investigative ophthalmology & visual science · 2024Article
- Ocular surface changes following vitreoretinal procedures.Indian journal of ophthalmology · 2023Review
- Biomedical Applications of Lactoferrin on the Ocular Surface.Pharmaceutics · 2023Review
- Dry eye disease in astronauts: a narrative review.Frontiers in physiology · 2023Review
- Transcorneal but not transpalpebral electrical stimulation disrupts mucin homeostasis of the ocular surface.BMC ophthalmology · 2022Article
- Human ocular mucins: The endowed guardians of sight.Advanced drug delivery reviews · 2022Review
- Characterisation of Gel-Forming Mucins Produced In Vivo and In Ex Vivo Conjunctival Explant Cultures.International journal of molecular sciences · 2021Article
- Characterization of the rabbit conjunctiva: Effects of sulfur mustard.Experimental and molecular pathology · 2021Article
- Membrane-associated mucins of the human ocular surface in health and disease.The ocular surface · 2021Review
- In the eye of the storm: SARS-CoV-2 infection and replication at the ocular surface?Stem cells translational medicine · 2021Review
- Short-Term Reproducibility of MUC5AC Measurement in Human Tear Fluid.Diagnostics (Basel, Switzerland) · 2021Article
- Dry Eye Etiology: Focus on Friction.Klinische Monatsblatter fur Augenheilkunde · 2020Article
- Biological functions of tear film.Experimental eye research · 2020Review
- Why Chain Length of Hyaluronan in Eye Drops Matters.Diagnostics (Basel, Switzerland) · 2020Review
- Immune - Goblet cell interaction in the conjunctiva.The ocular surface · 2020Review
- Membrane-associated mucins of the ocular surface: New genes, new protein functions and new biological roles in human and mouse.Progress in retinal and eye research · 2020Review
17 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Mucin genes, both secreted (MUC2, MUC5AC, MUC5B, MUC7) and membrane associated (MUC1, MUC4, MUC16), have been reported to be expressed by ocular surface epithelia. The purpose of this study was to comprehensively assay the mucin content of human tear fluid using multiple antibodies for each mucin and to develop a sensitive, semi-quantitative method for the assay of mucins in tears. Tear washes were obtained by instillation of saline onto the ocular surface, followed by collection from the inferior fornix. Tear proteins were separated in 1% agarose gels, transferred to nitrocellulose membrane by vacuum blotting and probed with multiple antibodies recognizing MUC1, MUC2, MUC4, MUC5AC, MUC5B, MUC7 and MUC16. Binding was detected using chemiluminescence, and quantity was determined by densitometry. Serial dilutions of pooled tears from normal individuals were assayed to determine the linear range of detectability. MUC1, MUC4, MUC16, MUC5AC and low levels of MUC2 were consistently detected in human tear fluid, while MUC5B and MUC7 were not. Use of several antibodies recognizing different epitopes on the same mucin confirmed these findings. The antibodies to mucins bound to serial dilutions of tears in a linear fashion (r2 > 0.9), indicating the feasibility of semi-quantitation. MUC5AC in tear fluid had an increased electrophoretic mobility compared to MUC5AC isolated from conjunctival tissue. This study provides clear evidence that the mucin component of tears is a mixture of secreted and shed membrane-associated mucins, and for the first time demonstrates MUC16 in tear fluid. Immunoblots of tears using agarose gel electrophoresis and chemiluminescence detection provide a semi-quantitative assay for mucin protein that will be useful for comparisons with tears from diseased eyes or after pharmacological intervention.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.