ArticleMutation research2007
Escherichia coli DNA polymerase IV contributes to spontaneous mutagenesis at coding sequences but not microsatellite alleles.
Article in Mutation research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 12 citations in OpenAlex.
- Sub-Minimum Inhibitory Concentrations of Rhubarb Water Extracts InhibitFrontiers in pharmacology · 2017Article
- A strategically located serine residue is critical for the mutator activity of DNA polymerase IV from Escherichia coli.Nucleic acids research · 2013Article
- Distinct mutational behaviors differentiate short tandem repeats from microsatellites in the human genome.Genome biology and evolution · 2013Article
- Revertant mosaicism in a human skin fragility disorder results from slipped mispairing and mitotic recombination.The Journal of clinical investigation · 2012Article
- Whole-genome analyses reveal genetic instability of Acetobacter pasteurianus.Nucleic acids research · 2009Article
- Every microsatellite is different: Intrinsic DNA features dictate mutagenesis of common microsatellites present in the human genome.Molecular carcinogenesis · 2009Review
- Human postmeiotic segregation 2 exhibits biased repair at tetranucleotide microsatellite sequences.Cancer research · 2009Article
- Stabilization of the genome of the mismatch repair deficient Mycobacterium tuberculosis by context-dependent codon choice.BMC genomics · 2008Article
- DNA polymerase kappa produces interrupted mutations and displays polar pausing within mononucleotide microsatellite sequences.Nucleic acids research · 2008Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Slipped strand mispairing during DNA synthesis is one proposed mechanism for microsatellite or short tandem repeat (STR) mutation. However, the DNA polymerase(s) responsible for STR mutagenesis have not been determined. In this study, we investigated the effect of the Escherichia colidinB gene product (Pol IV) on mononucleotide and dinucleotide repeat stability, using an HSV-tk gene episomal reporter system for microsatellite mutations. For the control vector (HSV-tk gene only) we observed a statistically significant 3.5-fold lower median mutation frequency in dinB(-) than dinB(+) cells (p<0.001, Wilcoxon Mann Whitney Test). For vectors containing an in-frame mononucleotide allele ([G/C](10)) or either of two dinucleotide alleles ([GT/CA](10) and [TC/AG](11)) we observed no statistically significant difference in the overall HSV-tk mutation frequency observed between dinB(+) and dinB(-) strains. To determine if a mutational bias exists for mutations made by Pol IV, mutational spectra were generated for each STR vector and strain. No statistically significant differences between strains were observed for either the proportion of mutational events at the STR or STR specificity among the three vectors. However, the specificity of mutational events at the STR alleles in each strain varied in a statistically significant manner as a consequence of microsatellite sequence. Our results indicate that while Pol IV contributes to spontaneous mutations within the HSV-tk coding sequence, Pol IV does not play a significant role in spontaneous mutagenesis at [G/C](10), [GT/CA](10), or [TC/AG](11) microsatellite alleles. Our data demonstrate that in a wild type genetic background, the major factor influencing microsatellite mutagenesis is the allelic sequence composition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.