ArticleAnalytical biochemistry2007
The development of quantum dot calibration beads and quantitative multicolor bioassays in flow cytometry and microscopy.
Article in Analytical biochemistry, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- High throughput analysis of rare nanoparticles with deep-enhanced sensitivity via unsupervised denoising.Nature communications · 2025Article
- Efficiency scale for scattering luminescent particles linked to fundamental and measurable spectroscopic properties.Scientific reports · 2023Article
- Optimisation of imaging flow cytometry for the analysis of single extracellular vesicles by using fluorescence-tagged vesicles as biological reference material.Journal of extracellular vesicles · 2019Article
- Dual-color immunofluorescent labeling with quantum dots of the diabetes-associated proteins aldose reductase and Toll-like receptor 4 in the kidneys of diabetic rats.International journal of nanomedicine · 2015Article
- Equilibrium and kinetics of Sin Nombre hantavirus binding at DAF/CD55 functionalized bead surfaces.Viruses · 2014Article
- Fluorogenic label to quantify the cytosolic delivery of macromolecules.Molecular bioSystems · 2013Article
- Investigation and development of quantum dot-encoded microsphere bioconjugates for DNA detection by flow cytometry.Journal of fluorescence · 2012Article
- Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots.Bioconjugate chemistry · 2011Article
- Quantum dots for quantitative flow cytometry.Methods in molecular biology (Clifton, N.J.) · 2011Article
- Recognition of decay accelerating factor and alpha(v)beta(3) by inactivated hantaviruses: Toward the development of high-throughput screening flow cytometry assays.Analytical biochemistry · 2010Article
- Real-time partitioning of octadecyl rhodamine B into bead-supported lipid bilayer membranes revealing quantitative differences in saturable binding sites in DOPC and 1:1:1 DOPC/SM/cholesterol membranes.The journal of physical chemistry. B · 2010Article
- Metal-Containing Polystyrene Beads as Standards for Mass Cytometry.Journal of analytical atomic spectrometry · 2010Article
- Calibration of Flow Cytometry for Quantitative Quantum Dot Measurements.Current protocols in cytometry · 2009Article
- QDs versus Alexa: reality of promising tools for immunocytochemistry.Journal of nanobiotechnology · 2009Article
- WebFlow: a software package for high-throughput analysis of flow cytometry data.Assay and drug development technologies · 2009Article
- Chapter 11. Subsecond analyses of G-protein coupled-receptor ternary complex dynamics by rapid mix flow cytometry.Methods in enzymology · 2009Article
- Potential use of quantum dots in flow cytometry.International journal of molecular sciences · 2008Article
- Flow cytometry for real-time measurement of guanine nucleotide binding and exchange by Ras-like GTPases.Analytical biochemistry · 2008Article
- Enhanced red and near infrared detection in flow cytometry using avalanche photodiodes.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2008Article
- Rapid-mix flow cytometry measurements of subsecond regulation of G protein-coupled receptor ternary complex dynamics by guanine nucleotides.Analytical biochemistry · 2007Article
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Authors and funding
6 authors.
Funding
Abstract
The use of fluorescence calibration beads has been the hallmark of quantitative flow cytometry. It has enabled the direct comparison of interlaboratory data as well as quality control in clinical flow cytometry. In this article, we describe a simple method for producing color-generalizable calibration beads based on streptavidin functionalized quantum dots. Based on their broad absorption spectra and relatively narrow emission, which is tunable on the basis of dot size, quantum dot calibration beads can be made for any fluorophore that matches their emission color. In an earlier publication, we characterized the spectroscopic properties of commercial streptavidin functionalized dots (Invitrogen). Here we describe the molecular assembly of these dots on biotinylated beads. The law of mass action is used to readily define the site densities of the dots on the beads. The applicability of these beads is tested against the industry standard, namely commercial fluorescein calibration beads. The utility of the calibration beads is also extended to the characterization surface densities of dot-labeled epidermal growth factor ligands as well as quantitative indicators of the binding of dot-labeled virus particles to cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.