ArticleBMC microbiology2007
Tandem repeat regions within the Burkholderia pseudomallei genome and their application for high resolution genotyping.
Article in BMC microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- The repertoire of short tandem repeats across the tree of life.Genome biology · 2025Article
- Highly specific and sensitive detection of Burkholderia pseudomallei genomic DNA by CRISPR-Cas12a.PLoS neglected tropical diseases · 2022Article
- Sequencing-Based Genotyping of PakistaniPathogens (Basel, Switzerland) · 2022Article
- Evolutionary Stability Optimizer (ESO): A Novel Approach to Identify and Avoid Mutational Hotspots in DNA Sequences While Maintaining High Expression Levels.ACS synthetic biology · 2022Article
- Genetic diversity and spatial distribution of Burkholderia mallei by core genome-based multilocus sequence typing analysis.PloS one · 2022Article
- Sequence-based detection and typing procedures forFrontiers in veterinary science · 2022Review
- Assessment of the control measures of the category A diseases of Animal Health Law:EFSA journal. European Food Safety Authority · 2022Article
- 16S rDNA and ITS Sequence Diversity of Burkholderia mallei Isolated from Glanders-Affected Horses and Mules in India (2013-2019).Current microbiology · 2021Article
- Molecular Characteristics ofFrontiers in microbiology · 2020Article
- An Evolutionary Arms Race BetweenFrontiers in microbiology · 2020Review
- Rapid antimicrobial susceptibility testing and β-lactam-induced cell morphology changes of Gram-negative biological threat pathogens by optical screening.BMC microbiology · 2018Article
- New Multilocus Variable-Number Tandem-Repeat Analysis (MLVA) Scheme for Fine-Scale Monitoring and Microevolution-Related Study of Ralstonia pseudosolanacearum Phylotype I Populations.Applied and environmental microbiology · 2017Article
- Molecular tracking investigation of melioidosis cases reveals regional endemicity in Hainan, China.Biomedical reports · 2016Article
- Genome Sequence of the Historical Clinical Isolate Burkholderia pseudomallei PHLS 6.Genome announcements · 2016Article
- Predicting the Genetic Stability of Engineered DNA Sequences with the EFM Calculator.ACS synthetic biology · 2015Article
- Tracing melioidosis back to the source: using whole-genome sequencing to investigate an outbreak originating from a contaminated domestic water supply.Journal of clinical microbiology · 2015Article
- Genotyping of Burkholderia mallei from an outbreak of glanders in Bahrain suggests multiple introduction events.PLoS neglected tropical diseases · 2014Article
- Multiple-locus variable number of tandem repeats analysis of Salmonella enterica serotype paratyphi A from Yuxi and comparison with isolates from the Chinese Medical Culture Collection Center.Molecular medicine reports · 2014Article
- Detailed structural analysis of the O-polysaccharide expressed by Burkholderia thailandensis E264.Carbohydrate research · 2012Article
- The genetic and molecular basis of O-antigenic diversity in Burkholderia pseudomallei lipopolysaccharide.PLoS neglected tropical diseases · 2012Article
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20 authors.
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Abstract
backgroundThe facultative, intracellular bacterium Burkholderia pseudomallei is the causative agent of melioidosis, a serious infectious disease of humans and animals. We identified and categorized tandem repeat arrays and their distribution throughout the genome of B. pseudomallei strain K96243 in order to develop a genetic typing method for B. pseudomallei. We then screened 104 of the potentially polymorphic loci across a diverse panel of 31 isolates including B. pseudomallei, B. mallei and B. thailandensis in order to identify loci with varying degrees of polymorphism. A subset of these tandem repeat arrays were subsequently developed into a multiple-locus VNTR analysis to examine 66 B. pseudomallei and 21 B. mallei isolates from around the world, as well as 95 lineages from a serial transfer experiment encompassing ~18,000 generations.
resultsB. pseudomallei contains a preponderance of tandem repeat loci throughout its genome, many of which are duplicated elsewhere in the genome. The majority of these loci are composed of repeat motif lengths of 6 to 9 bp with 4 to 10 repeat units and are predominately located in intergenic regions of the genome. Across geographically diverse B. pseudomallei and B.mallei isolates, the 32 VNTR loci displayed between 7 and 28 alleles, with Nei's diversity values ranging from 0.47 and 0.94. Mutation rates for these loci are comparable (>10-5 per locus per generation) to that of the most diverse tandemly repeated regions found in other less diverse bacteria.
conclusionThe frequency, location and duplicate nature of tandemly repeated regions within the B. pseudomallei genome indicate that these tandem repeat regions may play a role in generating and maintaining adaptive genomic variation. Multiple-locus VNTR analysis revealed extensive diversity within the global isolate set containing B. pseudomallei and B. mallei, and it detected genotypic differences within clonal lineages of both species that were identical using previous typing methods. Given the health threat to humans and livestock and the potential for B. pseudomallei to be released intentionally, MLVA could prove to be an important tool for fine-scale epidemiological or forensic tracking of this increasingly important environmental pathogen.
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