ArticleBritish journal of cancer1992
Enhancement of transforming growth factor-alpha synthesis in multicellular tumour spheroids of A431 squamous carcinoma cells.
Article in British journal of cancer, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Photoimmunotherapy: comparative effectiveness of two monoclonal antibodies targeting the epidermal growth factor receptor.Molecular oncology · 2014Article
- Spheroid-forming subpopulation of breast cancer cells demonstrates vasculogenic mimicry via hsa-miR-299-5p regulated de novo expression of osteopontin.Journal of cellular and molecular medicine · 2010Article
- Targeting a unique EGFR epitope with monoclonal antibody 806 activates NF-kappaB and initiates tumour vascular normalization.Journal of cellular and molecular medicine · 2009Article
- Cytoskeleton reorganization and ultrastructural damage induced by gliadin in a three-dimensional in vitro model.World journal of gastroenterology · 2005Article
- Replication of an integrin targeted conditionally replicating adenovirus on primary ovarian cancer spheroids.Cancer gene therapy · 2003Article
- The response of c-jun/AP-1 to chronic hypoxia is hypoxia-inducible factor 1 alpha dependent.Molecular and cellular biology · 2002Article
- Plasticity of the neoplastic phenotype in vivo is regulated by epigenetic factors.Proceedings of the National Academy of Sciences of the United States of America · 1998Article
- The molecular response of mammalian cells to hypoxia and the potential for exploitation in cancer therapy.The British journal of cancer. Supplement · 1996Review
- Massive programmed cell death in intestinal epithelial cells induced by three-dimensional growth conditions: suppression by mutant c-H-ras oncogene expression.The Journal of cell biology · 1995Article
- Drug and radiation resistance in spheroids: cell contact and kinetics.Cancer metastasis reviews · 1994Review
- Growth advantage ("clonal dominance") of metastatically competent tumor cell variants expressed under selective two- or three-dimensional tissue culture conditions.In vitro cellular & developmental biology. Animal · 1993Article
- Morphogenesis of "duct-like" structures in three-dimensional cultures of human cancerous pancreatic duct cells (Capan-1).In vitro cellular & developmental biology. Animal · 1993Article
- Acquired multicellular-mediated resistance to alkylating agents in cancer.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- TGF-beta gene expression depends on tissue architecture.In vitro cellular & developmental biology : journal of the Tissue Culture Association · 1993Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Multicellular tumour spheroids are cellular aggregates that can be prepared from many types of tumour cells. These three-dimensional structures provide a model for analysing the effects of cell-cell contact and intercellular microenvironments on phenomena such as autocrine regulation of growth factor synthesis. Autoregulation of the synthesis of transforming growth factor-alpha (TGF-alpha) was investigated at the message and protein levels in spheroid and monolayer cultures prepared from the A431 human squamous carcinoma cell line. The epidermal growth factor receptor (EGF-R) of these monolayer A431 cells had an average surface density of 2.2 x 10(6)/cell. Constitutive expression of TGF-alpha mRNA was an average of 3-fold greater in A431 spheroids than in monolayers, even for densely packed, confluent monolayers. This effect did not depend on hypoxic stress within the spheroids. TGF-alpha protein synthesis was enhanced in comparison with that in monolayer culture, reaching a value of up to 2-fold greater on a per cell basis. These results are discussed in the context of a TGF-alpha/EGF-R autocrine loop operating within cells that produce high local concentrations of TGF-alpha in the three-dimensional architecture of a spheroid.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.