ArticleFree radical biology & medicine2007
Regulation of MUC5AC expression by NAD(P)H:quinone oxidoreductase 1.
Article in Free radical biology & medicine, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 37 citations in OpenAlex.
- MMP12 deficiency attenuates menthol e-cigarette plus house dust-mite effects on pulmonary iron homeostasis and oxidative stress.Respiratory research · 2025Article
- Neutrophil elastase activates macrophage calpain as a mechanism for phagocytic failure.American journal of physiology. Lung cellular and molecular physiology · 2025Article
- Neutrophil Elastase and Chronic Lung Disease.Biomolecules · 2021Review
- Expression and Clinical Significance of Mucin Gene in Chronic Rhinosinusitis.Current allergy and asthma reports · 2020Review
- Identification of a Novel Inhibitor of Human Rhinovirus Replication and Inflammation in Airway Epithelial Cells.American journal of respiratory cell and molecular biology · 2019Article
- Inflammation, oxidative stress, and higher expression levels of Nrf2 and NQO1 proteins in the airways of women chronically exposed to biomass fuel smoke.Molecular and cellular biochemistry · 2018Article
- A small compound spindlactone A sensitizes human endometrial cancer cells to TRAIL-induced apoptosis via the inhibition of NAD(P)H dehydrogenase quinone 1.OncoTargets and therapy · 2018Article
- High-Mobility Group Box 1 Upregulates MUC5AC and MUC5B Expression in Primary Airway Epithelial Cells.American journal of respiratory cell and molecular biology · 2018Article
- COPD: balancing oxidants and antioxidants.International journal of chronic obstructive pulmonary disease · 2015Review
- Genome reference and sequence variation in the large repetitive central exon of human MUC5AC.American journal of respiratory cell and molecular biology · 2014Article
- Increased expression of senescence markers in cystic fibrosis airways.American journal of physiology. Lung cellular and molecular physiology · 2013Article
- NADPH:quinone oxidoreductase 1 regulates host susceptibility to ozone via isoprostane generation.The Journal of biological chemistry · 2013Article
- NAD(P)H quinone oxidoreductase 1 regulates neutrophil elastase-induced mucous cell metaplasia.American journal of physiology. Lung cellular and molecular physiology · 2012Article
- A candidate gene for autoimmune myasthenia gravis.Neurology · 2012Article
- RETRACTED: NAD(P)H:quinone oxidoreductase 1 protects lungs from oxidant-induced emphysema in mice.Free radical biology & medicine · 2012Article
- Role of hyaluronan and CD44 in reactive oxygen species-induced mucus hypersecretion.Molecular and cellular biochemistry · 2011Article
- Neutrophil elastase increases airway epithelial nonheme iron levels.Clinical and translational science · 2009Article
- NAD(P)H quinone oxidoreductase 1 is essential for ozone-induced oxidative stress in mice and humans.American journal of respiratory cell and molecular biology · 2009Article
- Proteases and cystic fibrosis.The international journal of biochemistry & cell biology · 2008Review
- Regulation of airway mucin gene expression.Annual review of physiology · 2008Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Neutrophil elastase (NE), a potent neutrophil inflammatory mediator, increases MUC5AC mucin gene expression through undefined pathways involving reactive oxygen species. To determine the source of NE-generated reactive oxygen species, we used pharmacologic inhibitors of oxidoreductases to test whether they blocked NE-regulated MUC5AC mRNA expression. We found that dicumarol, an inhibitor of the NADP(H):quinone oxidoreductase 1 (NQO1), inhibited MUC5AC mRNA expression in A549 lung adenocarcinoma cells and primary normal human bronchial epithelial cells. We further tested the role of NQO1 in mediating NE-induced MUC5AC expression by inhibiting NQO1 expression using short interfering RNA (siRNA). Transfection with siRNA specific for NQO1 suppressed NQO1 expression and significantly abrogated MUC5AC mRNA expression. NE treatment caused lipid peroxidation in A549 cells; this effect was inhibited by pretreatment with dicumarol, suggesting that NQO1 also regulates oxidant stress in A549 cells after NE exposure. NE exposure increased NQO1 protein and activity levels; NQO1 expression and activity were limited to the cytosol and did not translocate to the plasma membrane. Our results indicate that NQO1 has an important role as a key mediator of NE-regulated oxidant stress and MUC5AC mucin gene expression.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.