ArticleMolecular vision2007
Subfertility in mice harboring a mutation in betaB2-crystallin.
Article in Molecular vision, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- Physiological and pathological functions of βB2-crystallins in multiple organs: a systematic review.Aging · 2021Pooled it
- Analysis of mouse lens morphological and proteomic abnormalities following deletion of the βB3-crystallin promoter.Experimental eye research · 2025Article
- CRYβB2 alters cell adhesion to promote invasion in a triple-negative breast cancer cell line.BMC research notes · 2025Article
- A partial deletion within the meiosis-specific sporulation domain SPO22 of Tex11 is not associated with infertility in mice.PloS one · 2024Article
- Mouse models for microphthalmia, anophthalmia and cataracts.Human genetics · 2019Review
- Crybb2 Mutations Consistently Affect Schizophrenia Endophenotypes in Mice.Molecular neurobiology · 2019Article
- Transcriptome Analysis and Differential Gene Expression on the Testis of Orange Mud Crab, Scylla olivacea, during Sexual Maturation.PloS one · 2017Article
- Long non-coding RNAs regulate effects of β-crystallin B2 on mouse ovary development.Molecular medicine reports · 2016Article
- βA3/A1-crystallin: more than a lens protein.Progress in retinal and eye research · 2015Review
- Crybb2 coding for βB2-crystallin affects sensorimotor gating and hippocampal function.Mammalian genome : official journal of the International Mammalian Genome Society · 2013Article
- Article
- Clinical and experimental advances in congenital and paediatric cataracts.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Expression of βA3/A1-crystallin in the developing and adult rat eye.Journal of molecular histology · 2011Article
- Characterization of a novel mutation in the CRYBB2 gene associated with autosomal dominant congenital posterior subcapsular cataract in a Chinese family.Molecular vision · 2011Article
- Mouse models of cataract.Journal of genetics · 2009Review
- Congenital cataracts and their molecular genetics.Seminars in cell & developmental biology · 2008Review
- betaA3/A1-crystallin in astroglial cells regulates retinal vascular remodeling during development.Molecular and cellular neurosciences · 2008Article
- Mutation analysis in a German family identified a new cataract-causing allele in the CRYBB2 gene.Molecular vision · 2007Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposebetaB2-crystallin is one of the most abundant proteins of the adult ocular lens of mammals although it is expressed at lower levels in several extralenticular locations. While mutations in betaB2-crystallin are known to result in lens opacities, alterations in tissues besides the lens have not been previously investigated in these mutants. Since we found mice harboring the Crybb2Phil mutation bred poorly, here we assess the contribution of betaB2-crystallin to mouse fertility and determine the expression pattern of betaB2-crystallin in the testis.
methodsThe expression pattern of betaB2-crystallin in the testis was analyzed by rt-PCR, western blotting, and immunohistochemistry. The fecundity of wildtype and Crybb2Phil mice was analyzed by quantitative fertility testing. The morphology of testes and ovaries was assessed by hematoxylin and eosin staining.
resultsIn the mouse testis, betaB2-crystallin mRNA is found at low levels at birth, but its expression upregulates in this tissue as the testis is primed to initiate spermatogenesis. Western blotting detected betaB2-crystallin protein in sperm obtained from mice, cattle, and humans while immunolocalization detected this protein in developing sperm from the spermatocyte stage onward. Male and female mice homozygous for a 12 nucleotide inframe deletion mutation in betaB2-crystallin are subfertile when analyzed on a Swiss Webster derived background due to defects in egg and sperm production. However, mice harboring the same mutation on the C57Bl/6 genetic background did not exhibit any defects in reproductive function.
conclusionsbetaB2-crystallin is expressed in developing and mature sperm and mice of both sexes harboring the Philly mutation in the betaB2-crystallin gene are subfertile when analyzed on a Swiss Webster genetic background. While these data are suggestive of a role for betaB2-crystallin in fertility, definitive determination of this will await the creation of a betaB2-crystallin null mouse.
Indexed as
Identifiers
17392687PMC2642919W2207019662What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.