ArticleMolecular biology of the cell2007
RNAi knockdown of Nopp140 induces Minute-like phenotypes in Drosophila.
Article in Molecular biology of the cell, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Article
- Genetic Factors Linking Nucleolar Stress withInternational journal of molecular sciences · 2025Article
- Article
- A novel mistranslating tRNA model in Drosophila melanogaster has diverse, sexually dimorphic effects.G3 (Bethesda, Md.) · 2022Article
- Cell autonomous and non-autonomous consequences of deviations in translation machinery on organism growth and the connecting signalling pathways.Open biology · 2022Review
- Article
- Nucleolar stress inBiology open · 2020Article
- The Nopp140 gene in Drosophila melanogaster displays length polymorphisms in its large repetitive second exon.Molecular genetics and genomics : MGG · 2019Article
- Loss of Drosophila nucleostemin 2 (NS2) blocks nucleolar release of the 60S subunit leading to ribosome stress.Chromosoma · 2017Article
- Modeling congenital disease and inborn errors of development in Drosophila melanogaster.Disease models & mechanisms · 2016Review
- Deletion of Drosophila Nopp140 induces subcellular ribosomopathies.Chromosoma · 2015Article
- Mutation of a Nopp140 gene dao-5 alters rDNA transcription and increases germ cell apoptosis in C. elegans.Cell death & disease · 2014Article
- Conserved regulators of nucleolar size revealed by global phenotypic analyses.Science signaling · 2013Article
- A systematic RNAi synthetic interaction screen reveals a link between p53 and snoRNP assembly.Nature cell biology · 2011Article
- Knockdown of the Drosophila GTPase nucleostemin 1 impairs large ribosomal subunit biogenesis, cell growth, and midgut precursor cell maintenance.Molecular biology of the cell · 2009Article
- Nucleolar stress with and without p53.Nucleus (Austin, Tex.)Review
- Nucleolar stress in Drosophila melanogaster: RNAi-mediated depletion of Nopp140.Nucleus (Austin, Tex.)Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nopp140 associates with small nucleolar RNPs to chaperone pre-rRNA processing and ribosome assembly. Alternative splicing yields two isoforms in Drosophila: Nopp140-True is homologous to vertebrate Nopp140 particularly in its carboxy terminus, whereas Nopp140-RGG contains a glycine and arginine-rich (RGG) carboxy terminus typically found in vertebrate nucleolin. Loss of ribosome function or production at critical points in development leads to Minute phenotypes in Drosophila or the Treacher Collins syndrome (TCS) in humans. To ascertain the functional significance of Nopp140 in Drosophila development, we expressed interfering RNA using the GAL4/UAS system. Reverse transcription-PCR showed variable losses of Nopp140 mRNA in larvae from separate RNAi-expressing transgenic lines, whereas immunofluorescence microscopy with isoform-specific antibodies showed losses of Nopp140 in imaginal and polyploid tissues. Phenotypic expression correlated with the percent loss of Nopp140 transcripts: a >or=50% loss correlated with larval and pupal lethality, disrupted nuclear structures, and in some cases melanotic tumors, whereas a 30% loss correlated with adult wing, leg, and tergite deformities. We consider these adult phenotypes to be Minute-like and reminiscent of human craniofacial malformations associated with TCS. Similarly, overexpression of either isoform caused embryonic and larval lethality, thus indicating proper expression of Nopp140 is critical for normal development.
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