ArticleExperimental cell research2007
Transforming growth factor-beta1 regulates fibronectin isoform expression and splicing factor SRp40 expression during ATDC5 chondrogenic maturation.
Article in Experimental cell research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Alternative Splicing: A Critical Regulator in Human Bone Biology and Tumor Progression.Research (Washington, D.C.) · 2025Review
- EDA fibronectin-TLR4 axis sustains megakaryocyte expansion and inflammation in bone marrow fibrosis.The Journal of experimental medicine · 2019Article
- Effect of transforming growth factor-β2 on biological regulation of multilayer primary chondrocyte culture.Cell and tissue banking · 2018Article
- Introductory review: periostin-gene and protein structure.Cellular and molecular life sciences : CMLS · 2017Review
- TRPV4-dependent induction of a novel mammalian cold-inducible protein SRSF5 as well as CIRP and RBM3.Scientific reports · 2017Article
- Tissue stiffness regulates serine/arginine-rich protein-mediated splicing of the extra domain B-fibronectin isoform in tumors.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Mesenchymal stem cells promote a primitive phenotype CD34+c-kit+ in human cord blood-derived hematopoietic stem cells during ex vivo expansion.Cellular & molecular biology letters · 2013Article
- Article
- Splicosomal and serine and arginine-rich splicing factors as targets for TGF-β.Fibrogenesis & tissue repair · 2012Article
- Periostin in fibrillogenesis for tissue regeneration: periostin actions inside and outside the cell.Cellular and molecular life sciences : CMLS · 2011Review
- Differences between the early and advanced stages of rheumatoid arthritis in the expression of EDA-containing fibronectin.Rheumatology international · 2009Article
- Controlled differentiation of stem cells.Advanced drug delivery reviews · 2008Review
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
Abstract
Fibronectin (FN) isoform expression is altered during chondrocyte commitment and maturation, with cartilage favoring expression of FN isoforms that includes the type II repeat extra domain B (EDB) but excludes extra domain A (EDA). We and others have hypothesized that the regulated splicing of FN mRNAs is necessary for the progression of chondrogenesis. To test this, we treated the pre-chondrogenic cell line ATDC5 with transforming growth factor-beta1, which has been shown to modulate expression of the EDA and EDB exons, as well as the late markers of chondrocyte maturation; it also slightly accelerates the early acquisition of a sulfated proteoglycan matrix without affecting cell proliferation. When chondrocytes are treated with TGF-beta1, the EDA exon is preferentially excluded at all times whereas the EDB exon is relatively depleted at early times. This regulated alternative splicing of FN correlates with the regulation of alternative splicing of SRp40, a splicing factor facilitating inclusion of the EDA exon. To determine if overexpression of the SRp40 isoforms altered FN and FN EDA organization, cDNAs encoding these isoforms were overexpressed in ATDC5 cells. Overexpression of the long-form of SRp40 yielded an FN organization similar to TGF-beta1 treatment; whereas overexpression of the short form of SRp40 (which facilitates EDA inclusion) increased formation of long-thick FN fibrils. Therefore, we conclude that the effects of TGF-beta1 on FN splicing during chondrogenesis may be largely dependent on its effect on SRp40 isoform expression.
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