ArticleNucleic acids research2007
Real-time PCR mapping of DNaseI-hypersensitive sites using a novel ligation-mediated amplification technique.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed, 18 citations in OpenAlex.
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- The BAF (BRG1/BRM-Associated Factor) chromatin-remodeling complex exhibits ethanol sensitivity in fetal neural progenitor cells and regulates transcription at the miR-9-2 encoding gene locus.Alcohol (Fayetteville, N.Y.) · 2017Article
- Rapid and unambiguous detection of DNase I hypersensitive site in rare population of cells.PloS one · 2014Article
- CELDA -- an ontology for the comprehensive representation of cells in complex systems.BMC bioinformatics · 2013Article
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- Sequence composition similarities with the 7SL RNA are highly predictive of functional genomic features.Nucleic acids research · 2010Article
- Fine scale mapping of the breast cancer 16q12 locus.Human molecular genetics · 2010Article
- Rapid high-throughput analysis of DNaseI hypersensitive sites using a modified Multiplex Ligation-dependent Probe Amplification approach.BMC genomics · 2009Article
- FGFR2 variants and breast cancer risk: fine-scale mapping using African American studies and analysis of chromatin conformation.Human molecular genetics · 2009Article
- Context-dependent function of regulatory elements and a switch in chromatin occupancy between GATA3 and GATA2 regulate Gata2 transcription during trophoblast differentiation.The Journal of biological chemistry · 2009Article
- Article
- A novel mode of enhancer evolution: the Tal1 stem cell enhancer recruited a MIR element to specifically boost its activity.Genome research · 2008Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
Mapping sites within the genome that are hypersensitive to digestion with DNaseI is an important method for identifying DNA elements that regulate transcription. The standard approach to locating these DNaseI-hypersensitive sites (DHSs) has been to use Southern blotting techniques, although we, and others, have recently published alternative methods using a range of technologies including high-throughput sequencing and genomic array tiling paths. In this article, we describe a novel protocol to use real-time PCR to map DHS. Advantages of the technique reported here include the small cell numbers required for each analysis, rapid, relatively low-cost experiments with minimal need for specialist equipment. Presented examples include comparative DHS mapping of known TAL1/SCL regulatory elements between human embryonic stem cells and K562 cells.
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Registered trials
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