ArticleNucleic acids research2007
Trans-splicing of the Ll.LtrB group II intron in Lactococcus lactis.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 30 citations in OpenAlex.
- Mobile intron RNA from a bacterial predator accumulates in dead archaeal cells.Scientific reports · 2026Article
- Targeting the conserved active site of splicing machines with specific and selective small molecule modulators.Nature communications · 2024Article
- Molecular characterization of both transesterification reactions of the group II intron circularization pathway.Nucleic acids research · 2021Article
- Group II Introns Generate Functional Chimeric Relaxase Enzymes with Modified Specificities through Exon Shuffling at Both the RNA and DNA Level.Molecular biology and evolution · 2021Article
- The ChloroplastCells · 2021Review
- Therapeutic applications of trans-splicing.British medical bulletin · 2020Article
- Article
- Bacterial group II introns generate genetic diversity by circularization and trans-splicing from a population of intron-invaded mRNAs.PLoS genetics · 2018Article
- Recent horizontal transfer, functional adaptation and dissemination of a bacterial group II intron.BMC evolutionary biology · 2016Article
- Evolutionary Insights into RNA trans-Splicing in Vertebrates.Genome biology and evolution · 2016Review
- Circularization pathway of a bacterial group II intron.Nucleic acids research · 2016Article
- The Ll.LtrB intron from Lactococcus lactis excises as circles in vivo: insights into the group II intron circularization pathway.RNA (New York, N.Y.) · 2015Article
- Evolution of group II introns.Mobile DNA · 2015Article
- Bacterial cellular engineering by genome editing and gene silencing.International journal of molecular sciences · 2014Review
- Alternative splicing of a group II intron in a surface layer protein gene in Clostridium tetani.Nucleic acids research · 2014Article
- Isolation and characterization of functional tripartite group II introns using a Tn5-based genetic screen.PloS one · 2012Article
- Contribution of base-pairing interactions between group II intron fragments during trans-splicing in vivo.RNA (New York, N.Y.) · 2011Article
- Splicing of the Sinorhizobium meliloti RmInt1 group II intron provides evidence of retroelement behavior.Nucleic acids research · 2011Article
- Distribution, diversity, and potential mobility of extrachromosomal elements related to the Bacillus anthracis pXO1 and pXO2 virulence plasmids.Applied and environmental microbiology · 2009Article
- Trans-splicing versatility of the Ll.LtrB group II intron.RNA (New York, N.Y.) · 2008Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Ll.LtrB intron from the Gram-positive bacterium Lactococcus lactis is one of the most studied bacterial group II introns. Ll.LtrB interrupts the relaxase gene of three L. lactis conjugative elements. The relaxase enzyme recognizes the origin of transfer (oriT ) and initiates the intercellular transfer of its conjugative element. The splicing efficiency of Ll.LtrB from the relaxase transcript thus controls the conjugation level of its host element. Here, we used the level of sex factor conjugation as a read-out for Ll.LtrB splicing efficiency. Using this highly sensitive splicing/conjugation assay (10(7)-fold detection range), we demonstrate that Ll.LtrB can trans-splice in L. lactis when fragmented at various positions such as: three different locations within domain IV, within domain I and within domain III. We also demonstrate that the intron-encoded protein, LtrA, is absolutely required for Ll.LtrB trans-splicing. Characteristic Y-branched trans-spliced introns and ligated exons are detected by RT-PCR from total RNA extracts of cells harbouring fragmented Ll.LtrB. The splicing/conjugation assay we developed constitutes the first model system to study group II intron trans-splicing in vivo. Although only previously observed in bacterial-derived organelles, we demonstrate that assembly and trans-splicing of a fragmented group II intron can take place efficiently in bacterial cells.
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