ArticleProceedings of the National Academy of Sciences of the United States of America2007
Branch migration displacement assay with automated heuristic analysis for discrete DNA length measurement using DNA microarrays.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 16 citations in OpenAlex.
- Article
- Microarray-based STR genotyping using RecA-mediated ligation.Nucleic acids research · 2010Article
- Template tailoring: Accurate determination of heterozygous alleles using peptide nucleic acid and dideoxyNTP.Electrophoresis · 2010Article
- Analysis of short tandem repeats by parallel DNA threading.PloS one · 2009Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The analysis of short tandem repeats (STRs) plays an important role in forensic science, human identification, genetic mapping, and disease diagnostics. Traditional STR analysis utilizes gel- or column-based approaches to analyze DNA repeats. Individual STR alleles are separated and distinguished according to fragment length; thus the assay is generally hampered by its low multiplex capacity. However, use of DNA microarray would employ a simple hybridization and detection for field forensics and biology. Here we demonstrate a rapid, highly sensitive method for STR analysis that utilizes DNA microarray technology. We describe two adaptations to accomplish this: the use of competitive hybridization to remove unpaired ssDNA from an array and the use of neural network classification to automate the analysis. The competitive displacement technique mimics the branch migration process that occurs during DNA recombination. Our technique will facilitate the rapid deduction of identity, length, and number of repeats for the multiple STRs in an unknown DNA sample.
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