Evidence map›Paper›PMID 17389046›Full record

ArticleBMC genomics2007

Spotted cotton oligonucleotide microarrays for gene expression analysis.

Joshua A Udall, Lex E Flagel, Foo Cheung, Andrew W Woodward, Ran Hovav, Ryan A Rapp, Jordan M Swanson, Jinsuk J Lee, Alan R Gingle, Dan Nettleton and 3 more

Open access · goldAbstract read
In one paragraph

Article in BMC genomics, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
11.0field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 47 citations in OpenAlex.

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  4. The legacy of diploid progenitors in allopolyploid gene expression patterns.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 2 countries.

Joshua A UdallDepartment of Plant and Animal Sciences, Brigham Young University, Provo, UT 84062, USA. jaudall@byu.edu
Lex E Flagel
Foo Cheung
Andrew W Woodward
Ran Hovav
Ryan A Rapp
Jordan M Swanson
Jinsuk J Lee
Alan R Gingle
Dan Nettleton
Christopher D Town
Z Jeffrey Chen
Jonathan F Wendel
Iowa State University · USJ. Craig Venter Institute · USThe University of Texas at Austin · USBrigham Young University · USInstitut de Biologie Moléculaire et Cellulaire · FRUniversity of Georgia · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundMicroarrays offer a powerful tool for diverse applications plant biology and crop improvement. Recently, two comprehensive assemblies of cotton ESTs were constructed based on three Gossypium species. Using these assemblies as templates, we describe the design and creation and of a publicly available oligonucleotide array for cotton, useful for all four of the cultivated species.

resultsSynthetic oligonucleotide probes were generated from exemplar sequences of a global assembly of 211,397 cotton ESTs derived from >50 different cDNA libraries representing many different tissue types and tissue treatments. A total of 22,787 oligonucleotide probes are included on the arrays, optimized to target the diversity of the transcriptome and previously studied cotton genes, transcription factors, and genes with homology to Arabidopsis. A small portion of the oligonucleotides target unidentified protein coding sequences, thereby providing an element of gene discovery. Because many oligonucleotides were based on ESTs from fiber-specific cDNA libraries, the microarray has direct application for analysis of the fiber transcriptome. To illustrate the utility of the microarray, we hybridized labeled bud and leaf cDNAs from G. hirsutum and demonstrate technical consistency of results.

conclusionThe cotton oligonucleotide microarray provides a reproducible platform for transcription profiling in cotton, and is made publicly available through http://cottonevolution.info.

Indexed as

Gene Expression ProfilingOligonucleotide Array Sequence AnalysisExpressed Sequence TagsGenes, PlantGossypiumNucleic Acid Hybridization

Identifiers

PMID17389046
PMCPMC3225879
OpenAlexW1999439602

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.