ArticleMolecular biology reports2008
BcMF13, a new reproductive organ-specific gene from Brassica rapa. ssp. chinensis, affects pollen development.
Article in Molecular biology reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Identification of an anther-specific promoter from a male sterile AB line in Chinese cabbage (3 Biotech · 2022Article
- cDNA-AFLP analysis on bolting or flowering of flowering Chinese cabbage and molecular characteristics of BrcuDFR-like/BrcuAXS gene.Molecular biology reports · 2012Article
- Sequence characterization and expression pattern of BcMF21, a novel gene related to pollen development in Brassica campestris ssp. chinensis.Molecular biology reports · 2012Article
- Characterization of a new male sterility-related gene Camf1 in Capsicum annum L.Molecular biology reports · 2012Article
- BcMF20, a putative pollen-specific transcription factor from Brassica campestris ssp. chinensis.Molecular biology reports · 2011Article
- Isolation and characterization of a novel BcMF14 gene from Brassica campestris ssp. chinensis.Molecular biology reports · 2011Article
- A polygalacturonase inhibitory protein gene (BcMF19) expressed during pollen development in Chinese cabbage-pak-choi.Molecular biology reports · 2011Article
- Morphological and functional characterization of BcMF13 in the antisense-silenced plants of Brassica campestris ssp. chinensis var. parachinensis.Molecular biology reports · 2009Article
- The polygalacturonase gene BcMF2 from Brassica campestris is associated with intine development.Journal of experimental botany · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A transcript-derived fragment (GenBank accession number DN237920.1) accumulated in the wild-type flower buds of Chinese cabbage (Brassica campestris L. ssp. chinensis Makino, syn. B. rapa ssp. chinensis) was isolated and further investigated. The full length DNA and cDNA of the fragment were cloned by rapid amplification of cDNA ends. The gene, BcMF13, encodes a protein of 73 amino acids and is interrupted by an intron of 106 bp (GenBank accession number EF158459). Southern blot analysis revealed that BcMF13 could be a single-copy gene in the Chinese cabbage genome. Sequence blast analysis showed that BcMF13 was a new gene. In EST database, those sequences share 96-98% identity with BcMF13 cDNA all came from flower buds, microspores, anthers of Brassica, which proved BcMF13 homologs closely related to the development of male gametogenesis in Brassica. RT-PCR discovered that it is exclusively expressed in stage four and five flower buds of fertile line, strongly expressed in stamens. Successful suppression of BcMF13 gene expression by RNA antisense strategy greatly reduced the normal pollen grains, suggesting that BcMF13 was essential in pollen development in Brassica.
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