ArticleDigestive diseases and sciences2007
Taurodeoxycholate modulates apical Cl-/OH- exchange activity in Caco2 cells.
Article in Digestive diseases and sciences, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 50 citations in OpenAlex.
- Determining an optimal clinical dose of elobixibat, a novel inhibitor of the ileal bile acid transporter, in Japanese patients with chronic constipation: a phase II, multicenter, double-blind, placebo-controlled randomized clinical trial.Journal of gastroenterology · 2018Trial
- A randomized placebo-controlled phase IIb trial of a3309, a bile acid transporter inhibitor, for chronic idiopathic constipation.The American journal of gastroenterology · 2011Trial
- Chenodeoxycholate in females with irritable bowel syndrome-constipation: a pharmacodynamic and pharmacogenetic analysis.Gastroenterology · 2010Trial
- Effects of chenodeoxycholate and a bile acid sequestrant, colesevelam, on intestinal transit and bowel function.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2010Trial
- Real-world management of SeHCAT-confirmed bile acid diarrhoea: response to colestyramine and predictors of treatment success.Gastroenterology report · 2026Article
- Contributions of bile acids to gastrointestinal physiology as receptor agonists and modifiers of ion channels.American journal of physiology. Gastrointestinal and liver physiology · 2022Article
- Structural and Dynamic Determinants of Molecular Recognition in Bile Acid-Binding Proteins.International journal of molecular sciences · 2022Review
- The Role of Intestinal Permeability in Gastrointestinal Disorders and Current Methods of Evaluation.Frontiers in nutrition · 2021Review
- Altered metabolism of bile acids correlates with clinical parameters and the gut microbiota in patients with diarrhea-predominant irritable bowel syndrome.World journal of gastroenterology · 2020Article
- The Structure and Function of the Human Small Intestinal Microbiota: Current Understanding and Future Directions.Cellular and molecular gastroenterology and hepatology · 2020Review
- Biomaterial-tight junction interaction and potential impacts.Journal of materials chemistry. B · 2019Review
- Inhibition of CFTR-mediated intestinal chloride secretion as potential therapy for bile acid diarrhea.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Bile Acid Receptors and Gastrointestinal Functions.Liver research (Beijing, China) · 2019Article
- The Gut Microbiome in Adult and Pediatric Functional Gastrointestinal Disorders.Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association · 2019Review
- Modulating bile acid pathways and TGR5 receptors for treating liver and GI diseases.Current opinion in pharmacology · 2017Review
- Gut microbiome as a clinical tool in gastrointestinal disease management: are we there yet?Nature reviews. Gastroenterology & hepatology · 2017Review
- The Yin and Yang of bile acid action on tight junctions in a model colonic epithelium.Physiological reports · 2017Article
- Pathophysiology, Evaluation, and Management of Chronic Watery Diarrhea.Gastroenterology · 2017Review
- The Farnesoid X Receptor: Good for BAD.Cellular and molecular gastroenterology and hepatology · 2016Review
- Novel Therapies in IBS-D Treatment.Current treatment options in gastroenterology · 2015Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Bile acid malabsorption has been shown to be associated with diarrhea in cases such as ileal resection Crohn's disease of the ileum, and radiation enteritis. The mechanisms of bile acid-induced diarrhea are not fully understood. Although the induction of colonic chloride secretion in response to bile acids has been extensively investigated, to date the direct effect of bile acids on intestinal chloride absorption has not been well defined. Therefore, the current studies were undertaken to investigate the effect of bile acids on the apical Cl(-)/OH(-) exchange process utilizing Caco2 monolayers as an in vitro cellular model. Cl(-)/OH(-) exchange activity was measured as DIDS-sensitive pH gradient-driven (36)Cl uptake. The results are summarized as follows: (i) short-term exposure (20 min) of Caco2 cells to taurodeoxycholate (TDC; 200 microM) and glycochenodeoxycholate (GCDC; 200 microM) acids significantly inhibited apical Cl(-)/OH(-) exchange (by approximately 60-70%); (ii) the Ca(2+) chelator BAPTA-AM blocked the inhibition by TDC; (iii) the reduction in Cl(-)/OH(-) exchange by TDC was reversed by the PKC inhibitor, chelerythrine chloride; (iv) functional and inhibitor studies indicated that TDC induced inhibition of Cl(-)/OH(-) exchange was mediated via the activation of the PKC beta I isoform; (v) the effect of TDC on apical Cl(-)/OH(-) exchange was completely blocked by the PI3 kinase inhibitor LY294002 (5 microM); and (vi) the PKA inhibitor, RpcAMP, had no effect on TDC induced inhibition of Cl(-)/OH(-) exchange. In conclusion, our studies provide direct evidence for inhibition of human intestinal apical Cl(-)/OH(-) exchange activity by bile acids via Ca(2+)-, PI3 kinase-, and PKC beta I-dependent pathways in Caco2 cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.