ArticlePlanta2007
Tissue integrity and RNA quality of laser microdissected phloem of potato.
Article in Planta, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 39 citations in OpenAlex.
- BEL transcription factors in prominent Solanaceae crops: the missing pieces of the jigsaw in plant development.Planta · 2023Review
- Phloem Sap Composition: What Have We Learnt from Metabolomics?International journal of molecular sciences · 2023Review
- The interplay of DNA methyltransferases and demethylases with tuberization genes in potato (Frontiers in plant science · 2022Article
- Development of aerial and belowground tubers in potato is governed by photoperiod and epigenetic mechanism.Plant physiology · 2021Review
- Identification of phloem-associated translatome alterations during leaf development inHorticulture research · 2019Article
- The mobile RNAs, StBEL11 and StBEL29, suppress growth of tubers in potato.Plant molecular biology · 2017Article
- Transcriptional analysis of phloem-associated cells of potato.BMC genomics · 2015Article
- Identification of phloem-mobile mRNA.Journal of plant research · 2015Review
- MicroRNA156: a potential graft-transmissible microRNA that modulates plant architecture and tuberization in Solanum tuberosum ssp. andigena.Plant physiology · 2014Article
- The BEL1-like family of transcription factors in potato.Journal of experimental botany · 2014Article
- A perspective on photoperiodic phloem-mobile signals that control development.Frontiers in plant science · 2013Article
- Phloem-mobile messenger RNAs and root development.Frontiers in plant science · 2013Article
- The mRNA of a Knotted1-like transcription factor of potato is phloem mobile.Plant molecular biology · 2012Article
- Untranslated regions of a mobile transcript mediate RNA metabolism.Plant physiology · 2009Article
- Article
- Stamen abscission zone transcriptome profiling reveals new candidates for abscission control: enhanced retention of floral organs in transgenic plants overexpressing Arabidopsis ZINC FINGER PROTEIN2.Plant physiology · 2008Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The phloem is an important conduit for the transport of signaling molecules including RNA. Phloem sap has served as a source of RNA to profile uncontaminated phloem transcriptomes but its collection is difficult in many species. Laser capture microdissection techniques offer a valuable alternative for isolating RNA from specific vascular cells. In potato (Solanum tuberosum L.), there are seven BEL1-like transcription factors expressed throughout the plant with diverse functions. The RNA of one of these, StBEL5, moves through the phloem from the leaf to stolon tips to regulate tuber formation. In this study, the presence of several BEL RNAs and one Knotted1-like RNA was determined in phloem cells collected by laser microdissection coupled to laser pressure catapulting (LMPC). Three fixatives were compared for their effect on cell morphology and RNA quality in transverse sections of stems of potato. For optimum tissue integrity and quality of RNA from potato stem sections, the best results were achieved using ethanol acetate as the fixative. In addition, the RT-PCR results demonstrated the presence of six out of seven of the StBEL RNAs and a potato Knox RNA in phloem cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.