ArticleNucleic acids research1992
Processing of chimeric introns in dicot plants: evidence for a close cooperation between 5' and 3' splice sites.
Article in Nucleic acids research, 1992. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed.
- The AG dinucleotide terminating introns is important but not always required for pre-mRNA splicing in the maize endospermPlant physiology · 1999Article
- Use of alternate splice sites in granule-bound starch synthase mRNA from low-amylose rice varieties.Plant molecular biology · 1998Article
- Identification of protein-coding regions in Arabidopsis thaliana genome based on quadratic discriminant analysis.Plant molecular biology · 1998Article
- Enhanced expression in tobacco of the gene encoding green fluorescent protein by modification of its codon usage.Plant molecular biology · 1997Article
- Characterization of a novel arginine/serine-rich splicing factor in Arabidopsis.The Plant cell · 1996Article
- Splicing of precursors to mRNA in higher plants: mechanism, regulation and sub-nuclear organisation of the spliceosomal machinery.Plant molecular biology · 1996Review
- Splice site prediction in Arabidopsis thaliana pre-mRNA by combining local and global sequence information.Nucleic acids research · 1996Article
- Pre-mRNA splicing in plants: characterization of Ser/Arg splicing factors.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Characterization of intronic uridine-rich sequence elements acting as possible targets for nuclear proteins during pre-mRNA splicing in Nicotiana plumbaginifolia.Nucleic acids research · 1996Article
- Analysis of splice donor and acceptor site function in a transposable gene trap derived from the maize element Activator.Molecular & general genetics : MGG · 1995Article
- Efficient splicing of an AU-rich antisense intron sequence.Plant molecular biology · 1993Article
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2 authors.
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Abstract
Splice sites of vertebrate introns are generally not recognized in plant cells. Several lines of evidences have led to the proposal that the mechanism of 3' splice site selection differs in plants and animals (K. Wiebauer, J.J. Herrero, and W. Filipowicz, Mol. Cell. Biol. 8:2042-2051, 1988). To gain a better insight into the mechanistic differences between plant and animal splicing, we constructed chimeric introns consisting partly of dicotyledonous plant and partly of animal intron sequences. Splicing of these chimeric introns was analyzed in transiently transfected tobacco protoplasts. The results show that there are no principal sequence or structural differences between the 3' splice regions of plants and animals. Furthermore, evidence is provided that cooperation between 5' and 3' splice sites takes place and influences their mutual selection.
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