ArticleClinical and experimental nephrology2007
CCAAT/enhancer-binding protein-delta is induced in mesangial area during the early stages of anti-Thy1.1 glomerulonephritis and regulates cell proliferation and inflammatory gene expression in cultured rat mesangial cells.
Article in Clinical and experimental nephrology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 5 citations in OpenAlex.
- C/EBPδ Mediates Immunity to Renal Autoinflammatory Disorders in a Stage-specific Manner.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- CCAAT/Enhancer-Binding Proteins in Fibrosis: Complex Roles Beyond Conventional Understanding.Research (Washington, D.C.) · 2022Review
- Inflammation and renal fibrosis: Recent developments on key signaling molecules as potential therapeutic targets.European journal of pharmacology · 2018Review
- Rad GTPase inhibits cardiac fibrosis through connective tissue growth factor.Cardiovascular research · 2011Article
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4 authors at 1 institution in 1 country.
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Abstract
backgroundInterleukin (IL)-6, cyclooxygenase (COX)-2, and monocyte chemoattractant protein (MCP)-1 contribute to renal injury. The promoter regions of these genes contain CCAAT/enhancer-binding protein (C/EBP)-binding sites. In this study, we investigated the role of C/EBP-delta in mesangial cells (MCs).
methodsIn an in vivo study, anti-Thy 1.1 glomerulonephritis rats were generated and C/EBP-delta, IL-6, COX-2, and MCP-1 expressions were assessed by immunohistochemistry. In an in vitro study, cultured MCs were transfected with non-silencing (NS) short interfering RNA (siRNA) or C/EBP-delta siRNA. Subsequently, after stimulation with IL-1beta, C/EBP-delta, IL-6, COX-2, and MCP-1 mRNA expression levels were evaluated using real-time polymerase chain reaction (PCR). IL-6 concentration in the culture medium was determined by enzyme-linked immunosorbent assay. In addition, cell proliferative activity against IL-1beta or platelet-derived growth factor-BB was assessed by bromodeoxyuridine incorporation.
resultsIn the in vivo study, C/EBP-delta, IL-6, COX-2, and MCP-1 were expressed in the mesangial region of anti-Thy 1.1 glomerulonephritis rats on day 1. In the in vitro study, IL-1beta increased C/EBP-delta mRNA levels in NS siRNA-transfected MCs (7.3-fold), but no increase was evident in C/EBP-delta siRNA-transfected MCs. IL-6, COX-2, and MCP-1 mRNA levels in C/EBP-delta siRNA-transfected MCs were all lower than those in NS siRNA-transfected MCs (decreases of 57.7%, 85.7%, and 69.3%, respectively). The IL-6 concentration in the culture medium from C/EBP-delta siRNA transfected MCs (7.37 +/- 4.3 pg/ml) was also lower than that in the culture medium from NS siRNA-transfected MCs (25.2 +/- 3.4 pg/ml). Cell proliferative activity in C/EBP-delta siRNA-transfected MCs was lower than that in NS siRNA transfected MCs.
conclusionsC/EBP-delta was induced in the mesangial region during the early stages of anti-Thy1.1 glomerulonephritis. C/EBP-delta contributes to inflammatory gene expression and MC proliferation.
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