ArticleApplied and environmental microbiology2007
High-frequency phage-mediated gene transfer among Escherichia coli cells, determined at the single-cell level.
Article in Applied and environmental microbiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 45 citations in OpenAlex.
- Characterization of a novel phage-plasmid vB_EcoM_ED targeting multidrug-resistant Escherichia coli isolates.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Reduced ROS-associated prophage induction in a lepA mutant contributes to increased fluoroquinolone persistence in Salmonella Typhimurium.Scientific reports · 2026Article
- Integrated conjugative elements drive the formation of pandemic clones of Escherichia coli with hybrid chromosomes.Molecular biology and evolution · 2026Article
- Drugs in the environment, viruses in the balance: a review of the untold nexus of pharmaceuticals and virology.Frontiers in public health · 2026Review
- Stress-resistant but phage-sensitive host mutants induced by phage T4 ghost adsorption.Frontiers in microbiology · 2025Article
- A Critical Analysis of the Opportunities and Challenges of Phage Application in Seafood Quality Control.Foods (Basel, Switzerland) · 2024Review
- Interaction of bacteriophage P1 with an epiphyticFrontiers in microbiology · 2024Article
- Soil Component: A Potential Factor Affecting the Occurrence and Spread of Antibiotic Resistance Genes.Antibiotics (Basel, Switzerland) · 2023Review
- Horizontal Gene Transfer of Antibiotic Resistance Genes in Biofilms.Antibiotics (Basel, Switzerland) · 2023Review
- Bacterial Concentrations and Water Turbulence Influence the Importance of Conjugation Versus Phage-Mediated Antibiotic Resistance Gene Transfer in Suspended Growth Systems.ACS environmental Au · 2022Article
- Antibiotic Discovery and Resistance: The Chase and the Race.Antibiotics (Basel, Switzerland) · 2022Review
- Bacteriophages: Underestimated vehicles of antibiotic resistance genes in the soil.Frontiers in microbiology · 2022Review
- Prophage Activation in the Intestine: Insights Into Functions and Possible Applications.Frontiers in microbiology · 2021Review
- Cell-Free DNA: An Underestimated Source of Antibiotic Resistance Gene Dissemination at the Interface Between Human Activities and Downstream Environments in the Context of Wastewater Reuse.Frontiers in microbiology · 2020Review
- Viruses as key reservoirs of antibiotic resistance genes in the environment.The ISME journal · 2019Article
- Bacteriophages benefit from generalized transduction.PLoS pathogens · 2019Article
- Evolution of a Dominant Natural Isolate of Escherichia coli in the Human Gut over the Course of a Year Suggests a Neutral Evolution with Reduced Effective Population Size.Applied and environmental microbiology · 2018Article
- Bacteriophages Contribute to the Spread of Antibiotic Resistance Genes among Foodborne Pathogens of theFrontiers in microbiology · 2017Review
- Bacteriophages and genetic mobilization in sewage and faecally polluted environments.Microbial biotechnology · 2011Review
- Transfer of a phage T4 gene into Enterobacteriaceae, determined at the single-cell level.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent whole-genome analysis suggests that lateral gene transfer by bacteriophages has contributed significantly to the genetic diversity of bacteria. To accurately determine the frequency of phage-mediated gene transfer, we employed cycling primed in situ amplification-fluorescent in situ hybridization (CPRINS-FISH) and investigated the movement of the ampicillin resistance gene among Escherichia coli cells mediated by phage at the single-cell level. Phages P1 and T4 and the newly isolated E. coli phage EC10 were used as vectors. The transduction frequencies determined by conventional plating were 3x10(-8) to 2x10(-6), 1x10(-8) to 4x10(-8), and <4x10(-9) to 4x10(-8) per PFU for phages P1, T4, and EC10, respectively. The frequencies of DNA transfer determined by CPRINS-FISH were 7x10(-4) to 1x10(-3), 9x10(-4) to 3x10(-3), and 5x10(-4) to 4x10(-3) for phages P1, T4, and EC10, respectively. Direct viable counting combined with CPRINS-FISH revealed that more than 20% of the cells carrying the transferred gene retained their viabilities. These results revealed that the difference in the number of viable cells carrying the transferred gene and the number of cells capable of growth on the selective medium was 3 to 4 orders of magnitude, indicating that phage-mediated exchange of DNA sequences among bacteria occurs with unexpectedly high frequency.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.