ArticleThe Journal of molecular diagnostics : JMD2007
Development of an advanced electrochemical DNA biosensor for bacterial pathogen detection.
Article in The Journal of molecular diagnostics : JMD, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 110 citations in OpenAlex.
- A biosensor platform for rapid antimicrobial susceptibility testing directly from clinical samples.The Journal of urology · 2011Trial
- Harmful Microalgae Detection: Biosensors versus Some Conventional Methods.Sensors (Basel, Switzerland) · 2022Review
- Article
- Article
- Rapid Electrochemical-Based PCR-Less Microbial Quantification and Antimicrobial Susceptibility Profiling Directly From Blood and Urine With Unknown Microbial Load or Species.Frontiers in bioengineering and biotechnology · 2021Article
- Detection ofFoods (Basel, Switzerland) · 2019Review
- Molecular Techniques for the Detection of Organisms in Aquatic Environments, with Emphasis on Harmful Algal Bloom Species.Sensors (Basel, Switzerland) · 2017Review
- Integrated Biosensor Assay for Rapid Uropathogen Identification and Phenotypic Antimicrobial Susceptibility Testing.European urology focus · 2017Article
- Recent developments in detection and enumeration of waterborne bacteria: a retrospective minireview.MicrobiologyOpen · 2016Review
- Whole cell hybridisation for monitoring harmful marine microalgae.Environmental science and pollution research international · 2013Article
- Review: advances in electrochemical genosensors-based methods for monitoring blooms of toxic algae.Environmental science and pollution research international · 2013Review
- An AC electrokinetics facilitated biosensor cassette for rapid pathogen identification.The Analyst · 2013Article
- Bacterial detection & identification using electrochemical sensors.Journal of visualized experiments : JoVE · 2013Article
- Rapid antimicrobial susceptibility testing by sensitive detection of precursor rRNA using a novel electrochemical biosensing platform.Antimicrobial agents and chemotherapy · 2013Article
- Rapid, Affordable, and Point-of-Care Water Monitoring Via a Microfluidic DNA Sensor and a Mobile Interface for Global Health.IEEE journal of translational engineering in health and medicine · 2013Article
- Rapid hybridization of nucleic acids using isotachophoresis.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- In situ electrokinetic enhancement for self-assembled-monolayer-based electrochemical biosensing.Analytical chemistry · 2012Article
- Target-specific capture enhances sensitivity of electrochemical detection of bacterial pathogens.Journal of clinical microbiology · 2011Article
- Rapid detection of urinary tract infections using isotachophoresis and molecular beacons.Analytical chemistry · 2011Article
- Biosensor diagnosis of urinary tract infections: a path to better treatment?Trends in pharmacological sciences · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 3 institutions in 1 country.
Funding
Abstract
Electrochemical sensors have the capacity for rapid and accurate detection of a wide variety of target molecules in biological fluids. We have developed an electrochemical sensor assay involving hybridization of bacterial 16S rRNA to fluorescein-modified detector probes and to biotin-modified capture probes anchored to the sensor surface. Signal is generated by an oxidation-reduction current produced by the action of horseradish peroxidase conjugated to an anti-fluorescein monoclonal Fab. A previous study found that this electrochemical sensor strategy could identify uropathogens in clinical urine specimens. To improve assay sensitivity, we examined the key steps that affect the current amplitude of the electrochemical signal. Efficient lysis and release of 16S rRNA from both gram-negative and -positive bacteria was achieved with an initial treatment with Triton X-100 and lysozyme followed by alkaline lysis, resulting in a 12-fold increase in electrochemical signal compared with alkaline lysis alone. The distance in nucleotides between the target hybridization sites of the detector and capture probes and the location of fluorescein modification on the detector probe contributed to a 23-fold change in signal intensity. These results demonstrate the importance of target-probe and probe-probe interactions in the detection of bacterial 16S rRNA using an electrochemical DNA sensor approach.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.