ArticleJournal of bacteriology2007
Roles of His-rich hpn and hpn-like proteins in Helicobacter pylori nickel physiology.
Article in Journal of bacteriology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 80 citations in OpenAlex.
- Structure, dynamics and transferability of the metal-dependent polyhistidine tetramerization motif TetrHis for single-chain Fv antibodies.Communications chemistry · 2023Article
- A Comparative Study on Nickel Binding to Hpn-like Polypeptides from TwoInternational journal of molecular sciences · 2021Article
- A novel mode of control of nickel uptake by a multifunctional metallochaperone.PLoS pathogens · 2021Article
- Nickel chelation therapy as an approach to combat multi-drug resistant enteric pathogens.Scientific reports · 2019Article
- Acid-responsive activity of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Histidine tracts in human transcription factors: insight into metal ion coordination ability.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2018Article
- A novel mechanism of "metal gel-shift" by histidine-rich Ni2+-binding Hpn protein from Helicobacter pylori strain SS1.PloS one · 2017Article
- Characterization in Helicobacter pylori of a Nickel Transporter Essential for Colonization That Was Acquired during Evolution by Gastric Helicobacter Species.PLoS pathogens · 2016Article
- Article
- Evolution of Helicobacter: Acquisition by Gastric Species of Two Histidine-Rich Proteins Essential for Colonization.PLoS pathogens · 2015Article
- Aconitase Functions as a Pleiotropic Posttranscriptional Regulator in Helicobacter pylori.Journal of bacteriology · 2015Article
- Co-ordinate synthesis and protein localization in a bacterial organelle by the action of a penicillin-binding-protein.Molecular microbiology · 2013Article
- Inhibition of Helicobacter pylori urease activity in vivo by the synthetic nickel binding protein Hpn.European journal of microbiology & immunology · 2013Article
- Role of Helicobacter pylori methionine sulfoxide reductase in urease maturation.The Biochemical journal · 2013Article
- Helicobacter pylori stores nickel to aid its host colonization.Infection and immunity · 2013Article
- Common themes and unique proteins for the uptake and trafficking of nickel, a metal essential for the virulence of Helicobacter pylori.Frontiers in cellular and infection microbiology · 2013Review
- Helicobacter hepaticus NikR controls urease and hydrogenase activities via the NikABDE and HH0418 putative nickel import proteins.Microbiology (Reading, England) · 2013Article
- A supplemented soft agar chemotaxis assay demonstrates the Helicobacter pylori chemotactic response to zinc and nickel.Microbiology (Reading, England) · 2013Article
- Specific metal recognition in nickel trafficking.Biochemistry · 2012Review
- Detailed analysis of Helicobacter pylori Fur-regulated promoters reveals a Fur box core sequence and novel Fur-regulated genes.Molecular microbiology · 2012Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Individual gene-targeted hpn and hpn-like mutants and a mutant with mutations in both hpn genes were more sensitive to nickel, cobalt, and cadmium toxicity than was the parent strain, with the hpn-like strain showing the most metal sensitivity of the two individual His-rich protein mutants. The mutant strains contained up to eightfold more urease activity than the parent under nickel-deficient conditions, and the parent strain was able to achieve mutant strain activity levels by nickel supplementation. The mutants contained 3- to 4-fold more and the double mutant about 10-fold more Ni associated with their total urease pools, even though all of the strains expressed similar levels of total urease protein. Hydrogenase activities in the mutants were like those in the parent strain; thus, hydrogenase is fully activated under nickel-deficient conditions. The histidine-rich proteins appear to compete with the Ni-dependent urease maturation machinery under low-nickel conditions. Upon lowering the pH of the growth medium from 7.3 to 5, the wild-type urease activity increased threefold, but the activity in the three mutant strains was relatively unaffected. This pH effect was attributed to a nickel storage role for the His-rich proteins. Under low-nickel conditions, the addition of a nickel chelator did not significantly affect the urease activity of the wild type but decreased the activity of all of the mutants, supporting a role for the His-rich proteins as Ni reservoirs. These nickel reservoirs significantly impact the active urease activities achieved. The His-rich proteins play dual roles, as Ni storage and as metal detoxification proteins, depending on the exogenous nickel levels.
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Registered trials
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