ArticlePlant physiology2007
Combined transcriptome and proteome analysis identifies pathways and markers associated with the establishment of rapeseed microspore-derived embryo development.
Article in Plant physiology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Molecular Insights into Microspore Embryogenesis: Transcriptomic Dynamics in Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Establishment and maintenance of embryogenic cell fate during microspore embryogenesis.The Plant journal : for cell and molecular biology · 2025Article
- Transcriptomic identification of potential antioxidative enzyme regulators of the gametophytic-to-embryogenic switch in barley microspores.Frontiers in plant science · 2025Article
- The effect of phytosulfokine alpha on haploid embryogenesis and gene expression ofFrontiers in plant science · 2024Article
- Proteomic Analysis ofPlants (Basel, Switzerland) · 2023Article
- Review
- Transduction of Signals during Somatic Embryogenesis.Plants (Basel, Switzerland) · 2022Review
- Embryogenesis of European Radish (Plants (Basel, Switzerland) · 2021Article
- Live Imaging of embryogenic structures in Brassica napus microspore embryo cultures highlights the developmental plasticity of induced totipotent cells.Plant reproduction · 2020Article
- Differential Expression Profiling Reveals Stress-Induced Cell Fate Divergence in Soybean Microspores.Plants (Basel, Switzerland) · 2020Article
- Article
- Identification, Gene Structure, and Expression ofFrontiers in plant science · 2020Article
- Embryogenic competence of microspores is associated with their ability to form a callosic, osmoprotective subintinal layer.Journal of experimental botany · 2019Article
- Genetic Loci Governing Androgenic Capacity in Perennial Ryegrass (G3 (Bethesda, Md.) · 2018Article
- Differential Expression Profiling of Microspores During the Early Stages of Isolated Microspore Culture Using the Responsive Barley Cultivar Gobernadora.G3 (Bethesda, Md.) · 2018Article
- Global DNA methylation variations after short-term heat shock treatment in cultured microspores of Brassica napus cv. Topas.Scientific reports · 2016Article
- De novo assembly and characterization of global transcriptome of coconut palm (Cocos nucifera L.) embryogenic calli using Illumina paired-end sequencing.Protoplasma · 2016Article
- Effect of ovary induction on bread wheat anther culture: ovary genotype and developmental stage, and candidate gene association.Frontiers in plant science · 2015Article
- Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae.Frontiers in plant science · 2015Article
- The influence of heat stress on auxin distribution in transgenic B. napus microspores and microspore-derived embryos.Protoplasma · 2014Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microspore-derived embryo (MDE) cultures are used as a model system to study plant cell totipotency and as an in vitro system to study embryo development. We characterized and compared the transcriptome and proteome of rapeseed (Brassica napus) MDEs from the few-celled stage to the globular/heart stage using two MDE culture systems: conventional cultures in which MDEs initially develop as unorganized clusters that usually lack a suspensor, and a novel suspensor-bearing embryo culture system in which the embryo proper originates from the distal cell of a suspensor-like structure and undergoes the same ordered cell divisions as the zygotic embryo. Improved histodifferentiation of suspensor-bearing MDEs suggests a new role for the suspensor in driving embryo cell identity and patterning. An MDE culture cDNA array and two-dimensional gel electrophoresis and protein sequencing were used to compile global and specific expression profiles for the two types of MDE cultures. Analysis of the identities of 220 candidate embryo markers, as well as the identities of 32 sequenced embryo up-regulated protein spots, indicate general roles for protein synthesis, glycolysis, and ascorbate metabolism in the establishment of MDE development. A collection of 135 robust markers for the transition to MDE development was identified, a number of which may be coregulated at the gene and protein expression level. Comparison of the expression profiles of preglobular-stage conventional MDEs and suspensor-bearing MDEs identified genes whose differential expression may reflect improved histodifferentiation of suspensor-bearing embryos. This collection of early embryo-expressed genes and proteins serves as a starting point for future marker development and gene function studies aimed at understanding the molecular regulation of cell totipotency and early embryo development in plants.
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