ArticleCell & chromosome2007
Calyculin A, an enhancer of myosin, speeds up anaphase chromosome movement.
Article in Cell & chromosome, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Titin Is Present in the Elastic Tethers That Connect Separating Anaphase Chromosomes in Crane-Fly Spermatocytes.Cytoskeleton (Hoboken, N.J.) · 2026Article
- Article
- Structural evidence for elastic tethers connecting separating chromosomes in crane-fly spermatocytes.Life science alliance · 2023Article
- Centripetal nuclear shape fluctuations associate with chromatin condensation in early prophase.Communications biology · 2023Article
- Emergent collective organization of bone cells in complex curvature fields.Nature communications · 2023Article
- Blocking Protein Phosphatase 1 [PP1] Prevents Loss of Tether Elasticity in Anaphase Crane-Fly Spermatocytes.Frontiers in molecular biosciences · 2021Article
- The role of phosphorylation in the elasticity of the tethers that connect telomeres of separating anaphase chromosomes.Nucleus (Austin, Tex.) · 2020Article
- Evidence of Non-microtubule Spindle Forces inFrontiers in molecular biosciences · 2020Article
- Elastic Tethers Between Separating Anaphase Chromosomes Regulate the Poleward Speeds of the Attached Chromosomes in Crane-Fly Spermatocytes.Frontiers in molecular biosciences · 2020Article
- Strain-triggered mechanical feedback in self-organizing optic-cup morphogenesis.Science advances · 2018Article
- A review of "tethers": elastic connections between separating partner chromosomes in anaphase.Protoplasma · 2018Review
- Minocycline Promotes Neurite Outgrowth of PC12 Cells Exposed to Oxygen-Glucose Deprivation and Reoxygenation Through Regulation of MLCP/MLC Signaling Pathways.Cellular and molecular neurobiology · 2017Article
- MBTD1 is associated with Pr-Set7 to stabilize H4K20me1 in mouse oocyte meiotic maturation.Cell cycle (Georgetown, Tex.) · 2013Article
- Regional differences in actomyosin contraction shape the primary vesicles in the embryonic chicken brain.Physical biology · 2012Article
- A potential role for differential contractility in early brain development and evolution.Biomechanics and modeling in mechanobiology · 2012Article
- Flow mechanotransduction regulates traction forces, intercellular forces, and adherens junctions.American journal of physiology. Heart and circulatory physiology · 2012Article
- Do nuclear envelope and intranuclear proteins reorganize during mitosis to form an elastic, hydrogel-like spindle matrix?Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2011Review
- The perpetual movements of anaphase.Cellular and molecular life sciences : CMLS · 2010Review
- Rapid chromosome territory relocation by nuclear motor activity in response to serum removal in primary human fibroblasts.Genome biology · 2010Article
- Mitosis: spindle evolution and the matrix model.Protoplasma · 2009Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Actin and myosin inhibitors often blocked anaphase movements in insect spermatocytes in previous experiments. Here we treat cells with an enhancer of myosin, Calyculin A, which inhibits myosin-light-chain phosphatase from dephosphorylating myosin; myosin thus is hyperactivated. Calyculin A causes anaphase crane-fly spermatocyte chromosomes to accelerate poleward; after they reach the poles they often move back toward the equator. When added during metaphase, chromosomes at anaphase move faster than normal. Calyculin A causes prometaphase chromosomes to move rapidly up and back along the spindle axis, and to rotate. Immunofluorescence staining with an antibody against phosphorylated myosin regulatory light chain (p-squash) indicated increased phosphorylation of cleavage furrow myosin compared to control cells, indicating that calyculin A indeed increased myosin phosphorylation. To test whether the Calyculin A effects are due to myosin phosphatase or to type 2 phosphatases, we treated cells with okadaic acid, which inhibits protein phosphatase 2A at concentrations similar to Calyculin A but requires much higher concentrations to inhibit myosin phosphatase. Okadaic acid had no effect on chromosome movement. Backward movements did not require myosin or actin since they were not affected by 2,3-butanedione monoxime or LatruculinB. Calyculin A affects the distribution and organization of spindle microtubules, spindle actin, cortical actin and putative spindle matrix proteins skeletor and titin, as visualized using immunofluorescence. We discuss how accelerated and backwards movements might arise.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.