ArticleJournal of microscopy2007
Limited utility of acetoxymethyl (AM)-based intracellular delivery systems, in vivo: interference by extracellular esterases.
Article in Journal of microscopy, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 46 citations in OpenAlex.
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- Effects of Microencapsulated Ferulic Acid or Its Prodrug Methyl Ferulate on Neuroinflammation Induced by Muramyl Dipeptide.International journal of environmental research and public health · 2022Article
- Monitoring mitochondrial calcium and metabolism in the beating MCU-KO heart.Cell reports · 2021Article
- Challenges and Opportunities for Small-Molecule Fluorescent Probes in Redox Biology Applications.Antioxidants & redox signaling · 2018Review
- Cyanine Photocages Enable Spatial Control of Inducible Cre-Mediated Recombination.Chembiochem : a European journal of chemical biology · 2018Article
- A subset of extracellular vesicles carries the bulk of microRNAs in commercial dairy cow's milk.Journal of extracellular vesicles · 2017Article
- Deep insights: intravital imaging with two-photon microscopy.Pflugers Archiv : European journal of physiology · 2016Review
- Membrane-Permeable Mn(III) Complexes for Molecular Magnetic Resonance Imaging of Intracellular Targets.Journal of the American Chemical Society · 2016Article
- Use of independent component analysis to improve signal-to-noise ratio in multi-probe fluorescence microscopy.Journal of microscopy · 2014Article
- Dynamic multiphoton microscopy: focusing light on acute kidney injury.Physiology (Bethesda, Md.) · 2014Review
- Assessment of Plasmodium falciparum PfMDR1 transport rates using Fluo-4.Journal of cellular and molecular medicine · 2014Article
- In vivo microscopy reveals extensive embedding of capillaries within the sarcolemma of skeletal muscle fibers.Microcirculation (New York, N.Y. : 1994) · 2014Article
- In vivo multiphoton imaging of mitochondrial structure and function during acute kidney injury.Kidney international · 2013Article
- The first decade of using multiphoton microscopy for high-power kidney imaging.American journal of physiology. Renal physiology · 2012Review
- Rapid overlapping-volume acquisition and reconstruction (ROVAR): automated 3D tiling for high-resolution, large field-of-view optical microscopy.Journal of microscopy · 2011Article
- Multiphoton imaging of the functioning kidney.Journal of the American Society of Nephrology : JASN · 2011Article
- Minimally invasive molecular delivery into the brain using optical modulation of vascular permeability.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Multiphoton imaging of renal regulatory mechanisms.Physiology (Bethesda, Md.) · 2009Review
- A Nanocrystal-based Ratiometric pH Sensor for Natural pH Ranges.Chemical scienceArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The use of acetoxymethyl (AM) groups to deliver and trap exogenous optical probes inside cells is an established tool in cell biology/physiology, however, these probes have not been used extensively in vivo. In this study, the use of the acetoxymethyl delivery system for optical probes was evaluated, in vivo. Initial studies revealed very little trapped probe in intact tissues even when near saturating levels of probe were injected in living animals. We tested the hypothesis that extracellular esterases rapidly cleave the acetoxymethyl groups preventing the probes from entering cells, in vivo. The rates of hydrolysis of 11 acetoxymethyl probes in diluted porcine plasma revealed an essentially first order high rate dye cleavage with half times on the order of minutes or less. Studies on mice and rabbits revealed rates 10- to 2-fold higher, respectively. These plasma studies suggested that the acetoxymethyl probes were being cleaved before having a chance to enter cells in tissues in vivo. This was confirmed using intravital 2-photon excitation microscopy in muscle tissue where several acetoxymethyl probes were found to rapidly cleave in the vascular space during infusion and not be trapped in the muscle cells. Studies with succinimidyl esters that should quickly bind to proteins on cleavage also failed to enter cells, in vivo, consistent with the notion that the cleavage was occurring in the extracellular space. These data suggest that the high level of plasma and extracellular esterase activity render the classical acetoxymethyl probes ineffective for monitoring intracellular events, in vivo. Different approaches to trapping exogenous probes will need to be explored for physiological studies using intravital microscopy.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.