ArticleDifferentiation; research in biological diversity2007
Differentiation of cardiomyocytes requires functional serine residues within the amino-terminal domain of desmin.
Article in Differentiation; research in biological diversity, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Molecular insights into the role of desmin intermediate filament network in chromatin landscape, cardiomyocyte differentiation, and maturation.Cell death & disease · 2025Article
- Numb family proteins play roles in Desmin and Vimentin localization at the Z-disc.Journal of muscle research and cell motility · 2025Article
- A cytoplasmic escapee: desmin is going nuclear.Turkish journal of biology = Turk biyoloji dergisi · 2021Review
- Intermediate filaments in cardiomyopathy.Biophysical reviews · 2018Review
- Type III Intermediate Filaments Desmin, Glial Fibrillary Acidic Protein (GFAP), Vimentin, and Peripherin.Cold Spring Harbor perspectives in biology · 2017Review
- Desmin enters the nucleus of cardiac stem cells and modulates Nkx2.5 expression by participating in transcription factor complexes that interact with the nkx2.5 gene.Biology open · 2016Article
- Autophagic vacuolar pathology in desminopathies.Neuromuscular disorders : NMD · 2015Article
- Desmin related disease: a matter of cell survival failure.Current opinion in cell biology · 2015Review
- Phosphorylation of tropomodulin1 contributes to the regulation of actin filament architecture in cardiac muscle.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2014Article
- Μyospryn: a multifunctional desmin-associated protein.Histochemistry and cell biology · 2013Review
- Mechanisms of cardiogenesis in cardiovascular progenitor cells.International review of cell and molecular biology · 2012Review
- Self-organization phenomena in embryonic stem cell-derived embryoid bodies: axis formation and breaking of symmetry during cardiomyogenesis.Cells, tissues, organs · 2012Article
- Divide and conquer: the application of organelle proteomics to heart failure.Circulation research · 2011Review
- Desmin stimulates differentiation of cardiomyocytes and up-regulation of brachyury and nkx2.5.Differentiation; research in biological diversity · 2007Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Desmin contributes to the stability of the myocardium and its amino-terminal domain influences intermediate filament formation and interacts with a variety of proteins and DNAs. Specific serine residues located in this domain are reversibly phosphorylated in a cell cycle and developmental stage-dependent manner as has been demonstrated also for other cytoplasmic type III intermediate filament proteins. Although absence of desmin apparently does not affect cardiomyogenesis, homozygous deletion of the amino-terminal domain of desmin severely inhibited in vitro cardiomyogenesis. To demonstrate the significance of phosphorylation of this domain in cardiomyogenic commitment and differentiation, we inhibited phosphorylation of serine residues 6, 7, and 8 by mutation to alanine, and investigated early cardiomyogenesis in heterozygous embryoid bodies. As control, serine residues 31 and 32, which are not phosphorylated by kinases mutating serine residues 6, 7, and 8, were mutated to alanine in a second set. Desmin(S6,7,8A) interfered with cardiomyogenesis and myofibrillogenesis in a dominant negative fashion, whereas desmin(S31,32A) produced only a mild phenotype. Desmin(S6,7,8A) led to the down-regulation of the transcription factor genes brachyury, goosecoid, nkx2.5, and mef2C and increased apoptosis of presumptive mesoderm and differentiating cardiomyocytes. Surviving cardiomyocytes which were few in number had no myofibrils. Demonstration that some but not any mutant desmin interfered with the very beginning of cardiomyogenesis suggests an important function of temporarily phosphorylated serine residues 6, 7, and 8 in the amino-terminal domain of desmin in cardiomyogenic commitment and differentiation.
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