ArticleMolecular and cellular endocrinology2007
Egr-1 binds the GnRH promoter to mediate the increase in gene expression by insulin.
Article in Molecular and cellular endocrinology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Development of the gonadotropin-releasing hormone system.Journal of neuroendocrinology · 2022Review
- Ablating astrocyte insulin receptors leads to delayed puberty and hypogonadism in mice.PLoS biology · 2019Article
- Effects of Dietary Glucose on Serum Estrogen Levels and Onset of Puberty in Gilts.Asian-Australasian journal of animal sciences · 2016Article
- An Arf-Egr-C/EBPβ pathway linked to ras-induced senescence and cancer.Molecular and cellular biology · 2015Article
- Epigenetic DNA Methylation Linked to Social Dominance.PloS one · 2015Article
- Insulin receptor signaling in the GnRH neuron plays a role in the abnormal GnRH pulsatility of obese female mice.PloS one · 2015Article
- The regulation of reproductive neuroendocrine function by insulin and insulin-like growth factor-1 (IGF-1).Frontiers in neuroendocrinology · 2014Review
- Neuroendocrine mechanisms in athletes.Handbook of clinical neurology · 2014Review
- Delayed puberty but normal fertility in mice with selective deletion of insulin receptors from Kiss1 cells.Endocrinology · 2013Article
- Deletion of Otx2 in GnRH neurons results in a mouse model of hypogonadotropic hypogonadism.Molecular endocrinology (Baltimore, Md.) · 2011Article
- Divergent roles of growth factors in the GnRH regulation of puberty in mice.The Journal of clinical investigation · 2010Article
- Targeted deletion of somatotroph insulin-like growth factor-I signaling in a cell-specific knockout mouse model.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Regulation of gonadotropin-releasing hormone-1 gene transcription by members of the purine-rich element-binding protein family.American journal of physiology. Endocrinology and metabolism · 2010Article
- Impaired estrogen feedback and infertility in female mice with pituitary-specific deletion of estrogen receptor alpha (ESR1).Biology of reproduction · 2009Article
- Fasting-induced suppression of LH secretion does not require activation of ATP-sensitive potassium channels.American journal of physiology. Endocrinology and metabolism · 2008Article
- Phorbol 12-myristate 13-acetate (PMA) responsive sequence in Galphaq promoter during megakaryocytic differentiation. Regulation by EGR-1 and MAP kinase pathway.Thrombosis and haemostasis · 2008Article
- Development of an immortalised, post-pubertal gonadotrophin-releasing hormone neuronal cell line.Journal of neuroendocrinology · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Insulin increases gonadotropin-releasing hormone (GnRH) gene expression in in vitro models of GnRH neurons. Early growth response-1 (Egr-1) is a transcription factor that mediates the effect of insulin on target genes. In the GN11 cell line--an immortalized GnRH-secreting neuronal cell line--insulin maximally increases Egr-1 mRNA after 30min of treatment and Egr-1 protein and GnRH mRNA after 60min of treatment. Egr-1 small interfering RNA blocks the insulin-induced increase in GnRH promoter activity, measured as luciferase expression. Chromatin immunoprecipitation using Egr-1 antibody precipitates DNA in a proximal region of the GnRH promoter but not DNA in a distal region. Mutagenesis of a putative Egr-1 binding site within the proximal region blocks the insulin-induced increase in GnRH promoter activity. Thus, Egr-1 binds the GnRH promoter at a site between -67 and -76bp from the transcriptional start site to mediate the insulin-induced increase in GnRH gene transcription.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.