ArticleChemistry & biology2007
Diversification of catalytic function in a synthetic family of chimeric cytochrome p450s.
Article in Chemistry & biology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 71 citations in OpenAlex.
- Cytochrome P450The Journal of biological chemistry · 2025Article
- Development of a high throughput cytochrome P450 ligand-binding assay.The Journal of biological chemistry · 2024Article
- Choose Your Own Adventure: A Comprehensive Database of Reactions Catalyzed by Cytochrome P450 BM3 Variants.ACS catalysis · 2024Review
- A Continuing Career in Biocatalysis: Frances H. Arnold.ACS catalysis · 2019Article
- Understanding Flavin-Dependent Halogenase Reactivity via Substrate Activity Profiling.ACS catalysis · 2017Article
- High-throughput functional screening of steroid substrates with wild-type and chimeric P450 enzymes.BioMed research international · 2014Article
- SCHEMA computational design of virus capsid chimeras: calibrating how genome packaging, protection, and transduction correlate with calculated structural disruption.ACS synthetic biology · 2013Article
- Navigating the protein fitness landscape with Gaussian processes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- A divide-and-conquer approach to determine the Pareto frontier for optimization of protein engineering experiments.Proteins · 2012Article
- Recent advances in rational approaches for enzyme engineering.Computational and structural biotechnology journal · 2012Review
- Random field model reveals structure of the protein recombinational landscape.PLoS computational biology · 2012Article
- Exploring protein fitness landscapes by directed evolution.Nature reviews. Molecular cell biology · 2009Review
- Selective oxidation of carbolide C-H bonds by an engineered macrolide P450 mono-oxygenase.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A panel of cytochrome P450 BM3 variants to produce drug metabolites and diversify lead compounds.Chemistry (Weinheim an der Bergstrasse, Germany) · 2009Article
- In the light of directed evolution: pathways of adaptive protein evolution.Proceedings of the National Academy of Sciences of the United States of America · 2009Review
- Directed enzyme evolution: climbing fitness peaks one amino acid at a time.Current opinion in chemical biology · 2009Review
- Expanding the repertoire of biofuel alternatives through metabolic pathway evolution.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Neutral genetic drift can alter promiscuous protein functions, potentially aiding functional evolution.Biology direct · 2007Article
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5 authors at 1 institution in 1 country.
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Abstract
We report initial characterization of a synthetic family of more than 3000 cytochrome P450s made by SCHEMA recombination of 3 bacterial CYP102s. A total of 16 heme domains and their holoenzyme fusions with each of the 3 parental reductase domains were tested for activity on 11 different substrates. The results show that the chimeric enzymes have acquired significant functional diversity, including the ability to accept substrates not accepted by the parent enzymes. K-means clustering analysis of the activity data allowed the enzymes to be classified into five distinct groups based on substrate specificity. The substrates can also be grouped such that one can be a "surrogate" for others in the group. Fusion of a functional chimeric heme domain with a parental reductase domain always reconstituted a functional holoenzyme, indicating that key interdomain interactions are conserved upon reductase swapping.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.