ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2007
A mechanism intrinsic to the vesicle fusion machinery determines fast and slow transmitter release at a large CNS synapse.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed.
- Article
- Cellular and synaptic specializations for sub-millisecond precision in the mammalian auditory brainstem.Frontiers in cellular neuroscience · 2025Review
- Estimates of quantal synaptic parameters in light of more complex vesicle pool models.Frontiers in cellular neuroscience · 2025Article
- Tomosyns attenuate SNARE assembly and synaptic depression by binding to VAMP2-containing template complexes.Nature communications · 2024Article
- Article
- Article
- Three small vesicular pools in sequence govern synaptic response dynamics during action potential trains.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Influence of T-Bar on Calcium Concentration Impacting Release Probability.Frontiers in computational neuroscience · 2022Article
- A theory of synaptic transmission.eLife · 2021Article
- Article
- Direct Observation of Vesicle Transport on the Synaptic Ribbon Provides Evidence That Vesicles Are Mobilized and Prepared Rapidly for Release.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
- Differentially poised vesicles underlie fast and slow components of release at single synapses.The Journal of general physiology · 2020Article
- Rapid regulation of vesicle priming explains synaptic facilitation despite heterogeneous vesicle:CaeLife · 2020Article
- EGTA Can Inhibit Vesicular Release in the Nanodomain of Single CaFrontiers in synaptic neuroscience · 2019Article
- Developmental Increase of Neocortical Presynaptic EfficacyFrontiers in synaptic neuroscience · 2019Article
- Kinetics of transmitter release at the calyx of Held synapse.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2018Review
- Frequency-dependent mobilization of heterogeneous pools of synaptic vesicles shapes presynaptic plasticity.eLife · 2017Article
- Mechanisms of synaptic depression at the hair cell ribbon synapse that support auditory nerve function.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Some Subtle Lessons from the Calyx of Held Synapse.Biophysical journal · 2017Review
- Superpriming of synaptic vesicles as a common basis for intersynapse variability and modulation of synaptic strength.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
5 more citing papers are in PubMed but not listed here.
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Heterogeneity of release probability p between vesicles in the readily releasable pool (RRP) is expected to strongly influence the kinetics of depression at synapses, but the underlying mechanism(s) are not well understood. To test whether differences in the intrinsic Ca2+ sensitivity of vesicle fusion might cause heterogeneity of p, we made presynaptic Ca2+-uncaging measurements at the calyx of Held and analyzed the time course of transmitter release by EPSC deconvolution. Ca2+ uncaging, which produced spatially homogeneous elevations of [Ca2+]i, evoked a fast and a slow component of release over a wide range of [Ca2+]i, showing that mechanism(s) intrinsic to the vesicle fusion machinery cause fast and slow transmitter release. Surprisingly, the number of vesicles released in the fast component increased with Ca2+-uncaging stimuli of larger amplitudes, a finding that was most obvious below approximately 10 microM [Ca2+]i and that we call "submaximal release" of fast-releasable vesicles. During trains of action potential-like presynaptic depolarizations, submaximal release was also observed as an increase in the cumulative fast release at enhanced release probabilities. A model that assumes two separate subpools of RRP vesicles with different intrinsic Ca2+ sensitivities predicted the observed Ca2+ dependencies of fast and slow transmitter release but could not fully account for submaximal release. Thus, fast and slow transmitter release in response to prolonged [Ca2+]i elevations is caused by intrinsic differences between RRP vesicles, and an "a posteriori" reduction of the Ca2+ sensitivity of vesicle fusion after the onset of the stimulus might cause submaximal release of fast-releasable vesicles and contribute to short-term synaptic depression.
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