ArticleJournal of virology2007
Random screening for dominant-negative mutants of the cytomegalovirus nuclear egress protein M50.
Article in Journal of virology, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- Construction and Characterization of a High-Capacity Replication-Competent Murine Cytomegalovirus Vector for Gene Delivery.Vaccines · 2024Article
- Review
- High-throughput analysis of human cytomegalovirus genome diversity highlights the widespread occurrence of gene-disrupting mutations and pervasive recombination.Journal of virology · 2015Article
- Structure of a herpesvirus nuclear egress complex subunit reveals an interaction groove that is essential for viral replication.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Human cytomegalovirus UL97 phosphorylates the viral nuclear egress complex.Journal of virology · 2015Article
- Human cytomegalovirus UL50 and UL53 recruit viral protein kinase UL97, not protein kinase C, for disruption of nuclear lamina and nuclear egress in infected cells.Journal of virology · 2014Article
- A modified screening system for loss-of-function and dominant negative alleles of essential MCMV genes.PloS one · 2014Article
- Mapping of sequences in Pseudorabies virus pUL34 that are required for formation and function of the nuclear egress complex.Journal of virology · 2013Article
- Cytomegalovirus downregulates IRE1 to repress the unfolded protein response.PLoS pathogens · 2013Article
- Characterization of conserved region 2-deficient mutants of the cytomegalovirus egress protein pM53.Journal of virology · 2012Article
- Viral and host control of cytomegalovirus maturation.Trends in microbiology · 2012Review
- Article
- Cytomegalovirus replicon-based regulation of gene expression in vitro and in vivo.PLoS pathogens · 2012Article
- M94 is essential for the secondary envelopment of murine cytomegalovirus.Journal of virology · 2011Article
- Herpes simplex virus 1 pUL34 plays a critical role in cell-to-cell spread of virus in addition to its role in virus replication.Journal of virology · 2011Article
- Dominant negative mutants of the murine cytomegalovirus M53 gene block nuclear egress and inhibit capsid maturation.Journal of virology · 2010Article
- The m74 gene product of murine cytomegalovirus (MCMV) is a functional homolog of human CMV gO and determines the entry pathway of MCMV.Journal of virology · 2010Article
- Dominant-negative proteins in herpesviruses - from assigning gene function to intracellular immunization.Viruses · 2009Article
- Biochemical, biophysical, and mutational analyses of subunit interactions of the human cytomegalovirus nuclear egress complex.Journal of virology · 2009Article
- Tegument proteins of human cytomegalovirus.Microbiology and molecular biology reviews : MMBR · 2008Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Inactivation of gene products by dominant-negative (DN) mutants is a powerful tool to assign functions to proteins. Here, we present a two-step procedure to establish a random screen for DN alleles, using the essential murine cytomegalovirus gene M50 as an example. First, loss-of-function mutants from a linker-scanning library were tested for inhibition of virus reconstitution with the help of FLP-mediated ectopic insertion of the mutants into the viral genome. Second, DN candidates were confirmed by conditional expression of the inhibitory proteins in the virus context. This allowed the quantification of the inhibitory effect, the identification of the morphogenesis block, and the construction of DN mutants with improved activity. Based on these observations a DN mutant of the homologous gene (UL50) in human cytomegalovirus was predicted and constructed. Our data suggest that a proline-rich sequence motif in the variable region of M50/UL50 represents a new functional site which is essential for nuclear egress of cytomegalovirus capsids.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.