ArticleNucleic acids research2007
Reversible gene knockdown in mice using a tight, inducible shRNA expression system.
Article in Nucleic acids research, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Doublecortin-like knockdown in mice attenuates obesity by stimulating energy expenditure in adipose tissue.Scientific reports · 2024Article
- Krüppel-like factor 7 deficiency disrupts corpus callosum development and neuronal migration in the developing mouse cerebral cortex.Brain pathology (Zurich, Switzerland) · 2023Article
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- Suppression of Ribosomal Pausing by eIF5A Is Necessary to Maintain the Fidelity of Start Codon Selection.Cell reports · 2019Article
- A critical appraisal of the tafazzin knockdown mouse model of Barth syndrome: what have we learned about pathogenesis and potential treatments?American journal of physiology. Heart and circulatory physiology · 2019Review
- Generating mouse models for biomedical research: technological advances.Disease models & mechanisms · 2019Review
- Dose-dependent expression of claudin-5 is a modifying factor in schizophrenia.Molecular psychiatry · 2018Article
- Article
- Article
- The REMOTE-control system: a system for reversible and tunable control of endogenous gene expression in mice.Nucleic acids research · 2017Article
- Lgr6 is a stem cell marker in mouse skin squamous cell carcinoma.Nature genetics · 2017Article
- Overexpression of miR-210 and its significance in ischemic tissue damage.Scientific reports · 2017Article
- Article
- Inhibition of O-GlcNAcase leads to elevation of O-GlcNAc tau and reduction of tauopathy and cerebrospinal fluid tau in rTg4510 mice.Molecular neurodegeneration · 2017Article
- The PPAR pan-agonist bezafibrate ameliorates cardiomyopathy in a mouse model of Barth syndrome.Orphanet journal of rare diseases · 2017Article
- Individual Variation in Conditional β Cell Ablation Mice Contributes Significant Biases in Evaluating β Cell Functional Recovery.Frontiers in endocrinology · 2017Article
- Synthetic Tet-inducible small hairpin RNAs targeting hTERT or Bcl-2 inhibit malignant phenotypes of bladder cancer T24 and 5637 cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Potentiation of insulin-mediated glucose lowering without elevated hypoglycemia risk by a small molecule insulin receptor modulator.PloS one · 2015Article
- Article
- Genetically engineered mouse models for drug development and preclinical trials.Biomolecules & therapeutics · 2014Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RNA interference through expression of short hairpin (sh)RNAs provides an efficient approach for gene function analysis in mouse genetics. Techniques allowing to control time and degree of gene silencing in vivo, however, are still lacking. Here we provide a generally applicable system for the temporal control of ubiquitous shRNA expression in mice. Depending on the dose of the inductor doxycycline, the knockdown efficiency reaches up to 90%. To demonstrate the feasibility of our tool, a mouse model of reversible insulin resistance was generated by expression of an insulin receptor (Insr)-specific shRNA. Upon induction, mice develop severe hyperglycemia within seven days. The onset and progression of the disease correlates with the concentration of doxycycline, and the phenotype returns to baseline shortly after withdrawal of the inductor. On a broad basis, this approach will enable new insights into gene function and molecular disease mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.